Abstract | OBJECTIVE: To determine if antilens crystallin (ALC) serum and aqueous humor antibodies were present in normal dogs and dogs with cataracts, whether antibody incidence varied with stage of cataract, and whether antibody titer had a relationship to the presence of lens-induced uveitis. METHODS: RESULTS: SDS-PAGE identified bands with molecular weights of lens crystallin subunits. Western immunoblotting demonstrated reaction between canine serum and these protein bands. The five canine serum samples that reacted with crystallin subunits on Western blots had corresponding reactivity on the ELISA. All aqueous humor samples (30) were negative. Serum ALC antibodies were detected in 59.3% (16/27) of controls, 66.7% (16/24) of incipients, 50.0% (10/20) of immatures, 37.9% (11/29) of matures, 28.6% (6/21) of hypermatures, and 26.7% (4/15) of diabetics. Serum ALC antibodies were detected in 43.1% (47/109) of all cataract samples. There was a statistically significant negative association between the presence (P = 0.004) and maturity (P = 0.004) of cataract and presence of ALC serum antibodies. In the immature and hypermature cataract groups, there was a statistically significant negative association between ALC serum antibody titer and severity of uveitis (95% confidence interval). CONCLUSIONS: There is a negative association between the presence (P = 0.004) and maturity (P = 0.004) of cataract and presence of ALC serum antibodies.
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Authors | Heidi M Denis, Dennis E Brooks, Arthur R Alleman, Stacy E Andrew, Caryn Plummer |
Journal | Veterinary ophthalmology
(Vet Ophthalmol)
Vol. 6
Issue 4
Pg. 321-7
(Dec 2003)
ISSN: 1463-5216 [Print] England |
PMID | 14641830
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Autoantibodies
- Crystallins
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Topics |
- Animals
- Aqueous Humor
(metabolism)
- Autoantibodies
(analysis, blood, metabolism)
- Blotting, Western
(veterinary)
- Case-Control Studies
- Cataract
(immunology, veterinary)
- Crystallins
(immunology, isolation & purification)
- Dog Diseases
(blood, immunology)
- Dogs
- Electrophoresis, Polyacrylamide Gel
(veterinary)
- Enzyme-Linked Immunosorbent Assay
(veterinary)
- Female
- Lens, Crystalline
(immunology)
- Male
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