Blocking the T4 lysis inhibition phenotype.

Abstract

Nonlysogenic Escherichia coli K cells exhibit a delay in lysis when infected by T4rII phage termed lysis inhibition (LIN). E. coli K cells expressing lambda rexB from either a prophage defective for rexA, or a multicopy plasmid supported T4rII infection, but prevented the establishment of LIN. In addition, E. coli null mutations in either the periplasmic "tail-specific protease" tsp, or the 10Sa RNA ssrA, completely blocked the establishment of LIN following T4 infections. The expression of rexB in the absence of rexA resulted in several cellular phenotypes, including aberrant cell surface morphology, the partial to near complete suppression of mutations of lambda S and T4t holin genes, and lysis by cells aging on plates or growing with high rexB expression at elevated temperatures. These activities of RexB were impeded in the presence of RexA.

• Authors: Roderick A Slavcev, Sidney Hayes
• Journal: Gene (Gene) Vol. 321 Pg. 163-71 (Dec 04 2003) ISSN: 0378-1119 [Print] Netherlands
• PMID: 14637004 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Bacterial
• RexA protein, Bacteriophage lambda
• Viral Nonstructural Proteins
• Viral Proteins
• rexB protein, Bacteriophage lambda
• tmRNA
• Serine Endopeptidases
• Endopeptidase Clp
• Adenosine Triphosphatases

Topics

• Adenosine Triphosphatases (genetics, metabolism)
• Bacteriolysis (genetics)
• Bacteriophage T4 (genetics, growth & development)
• Endopeptidase Clp
• Escherichia coli (genetics, ultrastructure, virology)
• Gene Expression Regulation, Viral
• Lysogeny (genetics)
• Microscopy, Electron
• Mutation
• Phenotype
• RNA, Bacterial (genetics)
• Serine Endopeptidases (genetics, metabolism)
• Viral Nonstructural Proteins (genetics)
• Viral Proteins (genetics, metabolism)