Antithrombin reduction after experimental cardiopulmonary resuscitation.

Abstract

OBJECTIVE:

To determine whether activation of coagulation and inflammation during cardiac arrest results in a reduction of antithrombin (AT) and an increase in thrombin-antithrombin (TAT) complex during reperfusion.

METHODS:

Ventricular fibrillation (VF) was induced in ten anaesthetized pigs. After a 5-min non-intervention interval, closed-chest cardiopulmonary resuscitation (CPR) was performed for 9 min before defibrillation was attempted. If restoration of spontaneous circulation (ROSC) was achieved, the animals were observed for 4 h and repeated blood samples were taken for assay of AT, TAT and eicosanoids (8-iso-PGF(2alpha) and 15-keto-dihydro-PGF(2alpha)).

RESULTS:

AT began to decrease 15 min after ROSC and the reduction continued throughout the observation period (P<0.05). The lowest mean value (79%) occurred 60 min after ROSC. The TAT level was increased during the first 3 h after ROSC (P<0.05), indicating thrombin generation. The eicosanoids were increased throughout the observation period (P<0.05).

CONCLUSIONS:

AT is reduced and TAT and eicosanoids are increased after cardiac arrest, indicating activation of coagulation and inflammation.

• Authors: Jakob Johansson, Peter Ridefelt, Samar Basu, Sten Rubertsson
• Journal: Resuscitation (Resuscitation) Vol. 59 Issue 2 Pg. 235-42 (Nov 2003) ISSN: 0300-9572 [Print] Ireland
• PMID: 14625115 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antithrombins
• Biomarkers
• Dinoprost

Topics

• Analysis of Variance
• Animals
• Antithrombins (analysis, metabolism)
• Biomarkers (blood)
• Cardiopulmonary Resuscitation (methods)
• Dinoprost (blood)
• Disease Models, Animal
• Electric Countershock (methods)
• Female
• Hemodynamics (physiology)
• Male
• Myocardial Reperfusion
• Probability
• Radioimmunoassay
• Random Allocation
• Sensitivity and Specificity
• Swine
• Ventricular Fibrillation (therapy)