Abstract |
High molecular weight heat shock proteins (HSPs), hsp110 and grp170, derived from cancer cells have been previously shown to elicit tumor-specific immunity. This phenomenon is attributed to the antigenic peptides associated with the HSPs. Based on the unique chaperoning properties of these HSPs, a new vaccination strategy has been recently developed to elicit antigen-specific antitumor immunity. This approach utilizes tumor-associated antigens naturally complexed to these highly efficient molecular chaperones under heat shock conditions. This chapter focuses on the methodologies of these two vaccine strategies: I. purification of hsp110 and grp170 from tumor tissue or cell lines; II. generation and characterization of in vitro HSP- antigen complexes by heat shock using recombinant HSPs derived from a baculovirus protein expression system.
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Authors | Xiang-Yang Wang, Masoud H Manjili, Juneui Park, Xing Chen, Elizabeth Repasky, John R Subjeck |
Journal | Methods (San Diego, Calif.)
(Methods)
Vol. 32
Issue 1
Pg. 13-20
(Jan 2004)
ISSN: 1046-2023 [Print] United States |
PMID | 14624871
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Cancer Vaccines
- HSP110 Heat-Shock Proteins
- HSP70 Heat-Shock Proteins
- Heat-Shock Proteins
- Indicators and Reagents
- Recombinant Proteins
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Topics |
- Animals
- Cancer Vaccines
(isolation & purification)
- Chromatography, Affinity
(methods)
- Electrophoresis, Polyacrylamide Gel
(methods)
- Glycosylation
- HSP110 Heat-Shock Proteins
- HSP70 Heat-Shock Proteins
(immunology, isolation & purification)
- Heat-Shock Proteins
(immunology, isolation & purification)
- Immunoblotting
(methods)
- Indicators and Reagents
- Kinetics
- Mice
- Neoplasms
(immunology)
- Neoplasms, Experimental
(immunology)
- Protein Folding
- Recombinant Proteins
(immunology, isolation & purification)
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