An in vitro evaluation of the effects of homocysteine thiolactone on key steps of angiogenesis and tumor invasion.

Abstract	Homocysteine thiolactone is a highly reactive homocysteine derivative that can react easily with proteins. Protein homocysteinylation has been suggested as a possible mechanism underlying the pathological consequences of impaired homocysteine metabolism. Homocysteine inhibits key steps of angiogenesis and tumor invasion. It can be hypothesized that homocysteine thiolactone could mimic the described anti-angiogenic and anti-invasive effects of homocysteine. Therefore, we studied the effects of homocysteine thiolactone on different key steps of angiogenesis and tumor invasion, using model endothelial and tumor cell lines. This study demonstrates that homocysteine thiolactone, in high contrast to homocysteine, is not an anti-angiogenic compound. Furthermore, our results suggest that homocysteine thiolactone could behave as a pro-angiogenic compound.
Authors	Beatriz Martínez-Poveda, Teresa Chavarría, Francisca Sánchez-Jiménez, Ana R Quesada, Miguel Angel Medina
Journal	Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 311 Issue 3 Pg. 649-53 (Nov 21 2003) ISSN: 0006-291X [Print] United States
PMID	14623320 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Angiogenesis Inducing Agents Angiogenesis Inhibitors Culture Media, Conditioned Drug Combinations Laminin Proteoglycans Homocysteine matrigel Collagen homocysteine thiolactone Matrix Metalloproteinase 9
Topics	Angiogenesis Inducing Agents (pharmacology) Angiogenesis Inhibitors (pharmacology) Animals Aorta (pathology) Cattle Cell Differentiation Cell Division Cell Line, Tumor Cell Movement Collagen (pharmacology) Culture Media, Conditioned (pharmacology) Dose-Response Relationship, Drug Drug Combinations Endothelium, Vascular (cytology) Homocysteine (analogs & derivatives, chemistry, pharmacology) Humans In Vitro Techniques Laminin (pharmacology) Matrix Metalloproteinase 9 (metabolism) Neoplasm Invasiveness Neoplasms (drug therapy) Neovascularization, Pathologic Proteoglycans (pharmacology) Time Factors

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