Hyperosmolarity selectively enhances IgE-receptor-mediated histamine release from human basophils.

Abstract

Increased osmotic pressure has been reported to cause non-cytotoxic histamine release (HR) from human basophils, as well as a potentiation of HR induced by anti-IgE. In this study, the effects of hyperosmolar Na-K-acetate (300-600 mOsm/kg H2O) on HR was studied in washed human blood cells from newborns, adult volunteers and patients with severe atopic dermatitis. These three patient groups represented 3 very distinct populations with respect to total plasma IgE content, medians were less than 0.2 IU/ml, 20.5 IU/ml and 2508 IU/ml; respectively. Increasing osmolarity to 500 mOsm/kg H2O caused little HR in the absence of other stimuli, whereas at 600 mOsm/kg H2O a significant increase in spontaneous HR was seen. The HR induced by anti-IgE and Concanavalin A, acting through the IgE-receptor, was increased approximately twofold at 500 mOsm/kg H2O. Responses were highly correlated to results at 300 mOsm/kg H2O. The use of 600 mOsm/kg H2O buffers caused a further increase in most, but not all blood samples. The potentiation of IgE-receptor-mediated HR when using hyperosmolar media was clearly independent of plasma IgE contents, and did not change the concentration-response to anti-IgE. In contrast, HR induced by the IgE-receptor-independent stimuli, Formyl-met-leu-phe and calcium ionophore A 23187, were not enhanced at all by increased osmotic pressure. We conclude, that hyperosomolar media selectively enhance IgE-receptor-mediated HR. The use of hyperosmolar media may therefore be beneficial in a diagnostic application of washed blood HR assays used in allergy diagnosis.

• Authors: B W Nielsen, T Bjerke, T M Damsgaard, T Herlin, K Thestrup-Pedersen, P O Schiøtz
• Journal: Agents and actions (Agents Actions) Vol. 35 Issue 3-4 Pg. 170-8 (Mar 1992) ISSN: 0065-4299 [Print] Switzerland
• PMID: 1382367 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Acetates
• Alkanesulfonates
• Alkanesulfonic Acids
• Antigens, Differentiation, B-Lymphocyte
• Buffers
• Piperazines
• Receptors, Fc
• Receptors, IgE
• Immunoglobulin E
• piperazine-N,N'-bis(2-ethanesulfonic acid)

Topics

• Acetates
• Alkanesulfonates
• Alkanesulfonic Acids
• Antigens, Differentiation, B-Lymphocyte (physiology)
• Basophils (metabolism)
• Buffers
• Dermatitis, Atopic (blood)
• Fetal Blood (cytology, metabolism)
• Histamine Release (physiology)
• Humans
• Immunoglobulin E
• Osmolar Concentration
• Piperazines
• Receptors, Fc (physiology)
• Receptors, IgE
• Reference Values
• Reproducibility of Results