Abstract | BACKGROUND: METHODS: We studied pyloric tissue from nine infants with infantile hypertrophic pyloric stenosis and seven control infants and children. Cryostat sections were processed for NADPH diaphorase histochemical analysis. A polyclonal tau antiserum was used to identify the enteric nervous system by immunohistochemical methods. RESULTS:
NADPH diaphorase activity was restricted to the enteric nervous system and blood vessels. In the pyloric tissues from the control patients, intense diaphorase activity was present in the nerve fibers of the circular musculature, in the neurons and nerve bundles of the myenteric plexus, and in some nerve fibers of the longitudinal musculature. In the pyloric tissues from patients with infantile hypertrophic pyloric stenosis, the enteric nerve fibers in the hypertrophied circular musculature were enlarged and distorted and did not contain diaphorase activity, whereas the activity in the myenteric plexus and the longitudinal musculature was preserved. CONCLUSIONS:
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Authors | J M Vanderwinden, P Mailleux, S N Schiffmann, J J Vanderhaeghen, M H De Laet |
Journal | The New England journal of medicine
(N Engl J Med)
Vol. 327
Issue 8
Pg. 511-5
(Aug 20 1992)
ISSN: 0028-4793 [Print] United States |
PMID | 1378938
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Nitric Oxide Synthase
- Amino Acid Oxidoreductases
- NADPH Dehydrogenase
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Topics |
- Amino Acid Oxidoreductases
(metabolism)
- Child
- Child, Preschool
- Female
- Humans
- Hypertrophy
- Immunohistochemistry
- Infant
- Infant, Newborn
- Male
- Muscle Relaxation
- Myenteric Plexus
(enzymology)
- NADPH Dehydrogenase
(metabolism)
- Nitric Oxide Synthase
- Pyloric Stenosis
(enzymology, pathology, physiopathology)
- Pylorus
(enzymology, physiopathology)
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