Abstract |
In order to discriminate HTLV-II from HTLV-I, HTLV-II-specific polyclonal antibodies against a synthetic peptide of HTLV-II envelope sequence were raised in rabbits. We immunized two adult rabbits with a KLH-conjugated synthetic peptide corresponding to the amino acid sequence 171-196 of the HTLV-II envelope sequence, which is a specific region for HTLV-II as evaluated with an ELISA method. The resulting rabbit antisera to the synthetic peptide reacted with gp46 of HTLV-II lysates in Western blot analysis but not with that of HTLV-I. Flow cytometric analysis and immunohistochemical study revealed that these affinity purified antisera recognized some HTLV-II-producing cell lines examined, but not HTLV-I-producing cell lines or other cell lines uninfected by HTLV. These findings indicate that these antisera specifically recognized the envelope glycoprotein (gp46) of HTLV-II and suggest the specificity of this region in the immune response to HTLV-II. Such antisera are useful in distinguishing between HTLV-I and HTLV-II infection and in determining the presence of individual HTLV-II-infected cells both in vivo and in vitro, including non-lymphoid cells. They may also assist in the elucidation of the pathogenesis of HTLV-II.
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Authors | K Hayashi, N Ohara, K Fujiwara, H J Jeon, T R Koirala, K Takahashi, T Akagi, N Tomita, K Miyamoto |
Journal | Virchows Archiv. B, Cell pathology including molecular pathology
(Virchows Arch B Cell Pathol Incl Mol Pathol)
Vol. 63
Issue 1
Pg. 37-41
( 1992)
ISSN: 0340-6075 [Print] Germany |
PMID | 1362020
(Publication Type: Journal Article)
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Chemical References |
- Gene Products, env
- HTLV-I Antibodies
- HTLV-II Antibodies
- Retroviridae Proteins, Oncogenic
- env Gene Products, Human Immunodeficiency Virus
- gp46 protein, Human immunodeficiency virus 2
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Topics |
- Amino Acid Sequence
- Animals
- Gene Products, env
(genetics, immunology)
- HTLV-I Antibodies
(analysis, immunology)
- HTLV-II Antibodies
(analysis, immunology)
- Immunization
- Molecular Sequence Data
- Rabbits
- Retroviridae Proteins, Oncogenic
(genetics, immunology)
- env Gene Products, Human Immunodeficiency Virus
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