Simultaneous high-performance liquid chromatographic analysis of buspirone and its metabolite 1-(2-pyrimidinyl)-piperazine in plasma using electrochemical detection.

Abstract

A selective and sensitive high-performance liquid chromatographic method with coulometric detection is described for the quantitation of buspirone and its active metabolite, 1-(2-pyrimidinyl)piperazine, in plasma samples of mice treated orally with buspirone (10 mg/kg body weight). The analytes are extracted with a carboxylic acid solid-phase extraction column before chromatography. A dual-electrode electrochemical detector is used. The limit of detection is 50 pg for buspirone and 35 pg for 1-(2-pyrimidinyl)piperazine.

• Authors: P Betto, A Meneguz, G Ricciarello, S Pichini
• Journal: Journal of chromatography (J Chromatogr) Vol. 575 Issue 1 Pg. 117-21 (Mar 13 1992) Netherlands
• PMID: 1355487 (Publication Type: Journal Article)

Chemical References

• Anti-Anxiety Agents
• 1-(2-pyrimidinyl)piperazine
• Buspirone

Topics

• Administration, Oral
• Animals
• Anti-Anxiety Agents (blood)
• Buspirone (administration & dosage, analogs & derivatives, blood)
• Chromatography, High Pressure Liquid (methods)
• Electrochemistry
• Male
• Mice