An enhanced U6 promoter for synthesis of short hairpin RNA.

Abstract

Short hairpin RNAs (shRNAs) transcribed by RNA polymerase III (Pol III) promoters can trigger sequence-selective gene silencing in culture and in vivo and, therefore, may be developed to treat diseases caused by dominant, gain-of-function type of gene mutations. These diseases develop in people bearing one mutant and one wild-type gene allele. While the mutant is toxic, the wild-type performs important functions. Thus, the ideal therapy must selectively silence the mutant but maintain the wild-type expression. To achieve this goal, we designed an shRNA that selectively silenced a mutant Cu,Zn superoxide dismutase (SOD1(G93A)) allele that causes amyotrophic lateral sclerosis. However, the efficacy of this shRNA was relatively modest. Since the allele-specific shRNA has to target the mutation site, we could not scan other regions of SOD1 mRNA to find the best silencer. To overcome this problem, we sought to increase the dose of this shRNA by enhancing the Pol III promoter. Here we demonstrate that the enhancer from the cytomegalovirus immediate-early promoter can enhance the U6 promoter activity, the synthesis of shRNA and the efficacy of RNA interference (RNAi). Thus, this enhanced U6 promoter is useful where limited choices of shRNA sequences preclude the selection of a highly efficient RNAi target region.

• Authors: Xu Gang Xia, Hongxia Zhou, Hongliu Ding, El Bashir Affar, Yang Shi, Zuoshang Xu
• Journal: Nucleic acids research (Nucleic Acids Res) Vol. 31 Issue 17 Pg. e100 (Sep 01 2003) ISSN: 1362-4962 [Electronic] England
• PMID: 12930974 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Luminescent Proteins
• RNA, Messenger
• RNA, Small Interfering
• RNA, Small Nuclear
• U6 small nuclear RNA
• Green Fluorescent Proteins
• Superoxide Dismutase

Topics

• Base Sequence
• Blotting, Northern
• Blotting, Western
• Cell Line
• Cytomegalovirus (genetics)
• Enhancer Elements, Genetic (genetics)
• Gene Expression Regulation, Enzymologic
• Green Fluorescent Proteins
• Humans
• Luminescent Proteins (genetics, metabolism)
• Molecular Sequence Data
• Mutation
• Nucleic Acid Conformation
• Promoter Regions, Genetic (genetics)
• RNA Interference
• RNA, Messenger (genetics, metabolism)
• RNA, Small Interfering (chemistry, genetics, metabolism)
• RNA, Small Nuclear (genetics, metabolism)
• Superoxide Dismutase (genetics, metabolism)
• Transfection