Abstract | BACKGROUND: METHODS: RESULTS: Immunohistochemistry revealed that betaig-h3 occurs mainly in the basement membrane of proximal tubules, particularly the S3 segment but also to lesser extents in the basement membranes of the cortical thick ascending limb cells and the parietal glomerular epithelial cells in Bowman's capsule. Immunoblotting revealed that approximately 68 kD bands were seen only in the cortex + the outer stripe of the outer medulla. Rats with streptozotocin (STZ)-induced diabetes exhibited a marked and sustained increase in renal betaig-h3 abundance. This was mirrored by urinary betaig-h3 levels. In vitro experiments with human primary renal proximal tubular epithelial cells revealed that their expression of betaig-h3 was greatly increased by either TGF-beta or glucose. High glucose levels also stimulated TGF-beta production by renal proximal tubular epithelial cells and the high glucose-induced betaig-h3 expression was almost completely blocked by anti- TGF-beta antibody. betaig-h3 mediated renal proximal tubular epithelial cells adhesion and migration. CONCLUSION: betaig-h3 may be important in the development of diabetic nephropathy. Furthermore, the level of urinary betaig-h3 may be useful as an early marker reflecting disease onset and progression.
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Authors | Suk-Hee Lee, Jong-Sup Bae, Sun-Hee Park, Byung-Heon Lee, Rang-Woon Park, Je-Yong Choi, Jae Yong Park, Sung-Woo Ha, Yong-Lim Kim, Tae-Hwan Kwon, In-San Kim |
Journal | Kidney international
(Kidney Int)
Vol. 64
Issue 3
Pg. 1012-21
(Sep 2003)
ISSN: 0085-2538 [Print] United States |
PMID | 12911551
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Extracellular Matrix Proteins
- Transforming Growth Factor beta
- betaIG-H3 protein
- Glucose
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Topics |
- Animals
- Cell Adhesion
- Cell Movement
- Cells, Cultured
- Diabetes Mellitus, Experimental
(metabolism)
- Dose-Response Relationship, Drug
- Epithelial Cells
(metabolism, physiology)
- Extracellular Matrix Proteins
(metabolism, urine)
- Glucose
(administration & dosage)
- Humans
- Immunologic Techniques
- Kidney
(metabolism)
- Kidney Tubules, Proximal
(metabolism, physiology)
- Male
- Rats
- Rats, Sprague-Dawley
- Tissue Distribution
- Transforming Growth Factor beta
(metabolism, physiology, urine)
- Up-Regulation
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