[Effect of Tripterygium Wilfordii Hook T4 monomer on proliferation and interleukin-6 production of synovial fibroblasts of patients with rheumatoid arthritis].

Abstract

OBJECTIVE:

Tripterygium Wilfordii Hook multi-glycosides T2 has been wildly used in China in treatment of RA. T4 was abstracted from T2 and was reported much more efficient in anti-inflammatory and immune suppression than T2. This study was to investigate the effect Tripterygium Wilfordii Hook T4 monomer on proliferation and interleukin-6 production of synovial fibroblasts of patients with rheumatoid arthritis.

METHODS:

Synovium was obtained from patients with rheumatoid arthritis undergoing synovectomies or joint replacement. Cultures of synovial fibroblasts were established. After 3 generations, cultured synovial fibroblasts were stimulated with IL-1. Then 1.5 ng/ml, 5 ng/ml and 15 ng/ml T4 were added, and synovial fibroblasts were cultured in the presence of T4 for 48 hours. Cell proliferation was assayed using MTT method. IL-6 level of supernatant was measured by ELISA.

RESULTS:

Proliferation of synovial fibroblasts was inhibited by T4. The proliferation inhibition effect of T4 was dose dependent and inhibition rate was 5.18%, 10.95% and 21.37%, respectively. And T4 had no effect on IL-6 production by IL-1 stimulated synovial fibroblasts.

CONCLUSIONS:

T4 might control the disease activity of RA by inhibiting the proliferation of synoviocyte. And T4 might not influence the concentration of IL-6 in synovial fluid, as a central effect, since IL-6 has protective effect on articular cartilage.

• Authors: Y Guo, M Yu, Y Jiang, Q Song, Y Dong
• Journal: Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae (Zhongguo Yi Xue Ke Xue Yuan Xue Bao) Vol. 22 Issue 2 Pg. 190-2 (Apr 2000) ISSN: 1000-503X [Print] China
• PMID: 12903526 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Diterpenes
• Interleukin-1
• Interleukin-6
• Phenanthrenes
• tripchlorolide

Topics

• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Arthritis, Rheumatoid (pathology)
• Cell Division (drug effects)
• Cells, Cultured
• Diterpenes (pharmacology)
• Drug Antagonism
• Female
• Fibroblasts (metabolism)
• Humans
• Interleukin-1 (pharmacology)
• Interleukin-6 (biosynthesis)
• Male
• Middle Aged
• Phenanthrenes (pharmacology)
• Synovial Membrane (cytology)
• Tripterygium (chemistry)