[Role of interleukin-2 in the functional myocardial impairment induced by anoxia and reoxygenation].

Abstract

OBJECTIVE:

To investigate the effect of interleukin-2 (IL-2) on myocardial impairment during ischemia/reperfusion or anoxia/reoxygenation.

METHODS:

Chemical anoxia was introduced in the isolated rat ventricular myocytes by Krebs-Henseleit (K-H) solution containing 10(-3) mol/L sodium dithionite. The video-tracking system and spectrofluorometric method were employed to verify the cell contraction and calcium homeostasis of the single myocyte. Radioimmunoassay was used to analyze the IL-2 levels in myocardium.

RESULTS:

The levels of IL-2 in myocardium subjected to ischemia/reperfusion were elevated [(14.34+/-5.99 compared with 22.25+/-3.68)ng/g, P<0.01]. During anoxia, cell contraction and the amplitude of electrically induced calcium transient were depressed and the parameters did not return to the pre-anoxia level during reoxygenation. IL-2 at 200 U/L administered during anoxia aggravated the effect of reoxygenation on cell contraction and calcium transient. After perfusion with IL-2, the malondialdehyde content of myocardial mitochondria was elevated.

CONCLUSION:

Coexistence of IL-2 during anoxia aggravates the effect of reoxygenation on the cell contraction and calcium homeostasis in the isolated rat ventricular myocytes, in which the mitochondrial lipid peroxidation induced by IL-2 is involved.

• Authors: Chun-mei Cao, Hui Yao, Wan-hong Xu, Zhi-guo Ye, Jun-zhu Chen, Qiang Xia
• Journal: Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences (Zhejiang Da Xue Xue Bao Yi Xue Ban) Vol. 32 Issue 3 Pg. 175-80 (06 2003) ISSN: 1008-9292 [Print] China
• PMID: 12881858 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-2
• Malondialdehyde
• Calcium

Topics

• Animals
• Calcium (metabolism)
• Interleukin-2 (physiology)
• Lipid Peroxidation
• Male
• Malondialdehyde (blood)
• Mitochondria, Heart (metabolism)
• Myocardial Ischemia (physiopathology)
• Myocardial Reperfusion Injury (physiopathology)
• Rats
• Rats, Sprague-Dawley