[Expression of vascular endothelial growth factor and its receptors KDR and Flt1 in acute myeloid leukemia].

Abstract

OBJECTIVE:

To evaluate the expression of vascular endothelial growth factor (VEGF) and its receptors KDR and Flt1 in patients with acute myeloid leukemia (AML).

METHODS:

The expression of VEGF and its receptors mRNA was assayed by RT-PCR, the plasma of VEGF by ELISA.

RESULTS:

In 13 AML cell lines, the expression of VEGF, KDR and Flt1 mRNA were found in 13 (100%), 7 (53.8%) and 12 (92.3%), respectively. There were 21 (65.6%), 1 (3.1%), and 17 (53.1%) of 31 (AML) patients bone marrow mononuclear cells (BMMNCs) expressing VEGF, KDR and Flt1 mRNA, respectively. None of BMMNCs from 3 normal donor and CD(34)(+) cells from 2 normal donor was found to express VEGF, KDR and Flt1 mRNA. The plasma level of VEGF of 39 patients (new diagnosed, relapsed and secondary-AML) before treatment was (135.3 +/- 87.9) ng/L which was significantly higher than that of 15 complete remission (CR) patients (80.6 +/- 36.9) ng/L and 12 normal donors (80.6 +/- 33.1) micro g/L (P = 0.028, 0.007). The plasma level of VEGF of 15 non-responsive patients was (188.2 +/- 118.6) ng/L after two cycles of chemotherapy which was higher than that of 20 CR patients [(104.2 +/- 30.9) ng/L] (P = 0.004).

CONCLUSION:

VEGF and its receptors KDR and Flt1 mRNAs were expressed in BMMNCs of AML patients. The plasma level of VEGF directly affected the response to chemotherapy in AML patients.

• Authors: Yi Wang, Zhi-jian Xiao, Peng Liu, Chen Yang, Ren-chi Yang, Ying-lin Cai, Zhong-chao Han
• Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi (Zhonghua Xue Ye Xue Za Zhi) Vol. 24 Issue 5 Pg. 249-52 (May 2003) ISSN: 0253-2727 [Print] China
• PMID: 12859876 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Messenger
• Vascular Endothelial Growth Factor A
• FLT1 protein, human
• Vascular Endothelial Growth Factor Receptor-1
• Vascular Endothelial Growth Factor Receptor-2

Topics

• Enzyme-Linked Immunosorbent Assay
• Humans
• Leukemia, Myeloid, Acute (drug therapy, metabolism)
• RNA, Messenger (genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• Vascular Endothelial Growth Factor A (biosynthesis, genetics)
• Vascular Endothelial Growth Factor Receptor-1 (biosynthesis, genetics)
• Vascular Endothelial Growth Factor Receptor-2 (biosynthesis, genetics)