Human herpesvirus 6 infection of human epidermal cell line: pathogenesis of skin manifestations.

Abstract

In order to elucidate the pathogenesis of variant B human herpesvirus 6 (HHV-6) infection in skin tissues, an A431 cell line was inoculated with variant B HHV-6. HHV-6 causes abortive infection in the A431 cells, because neither late antigen (OHV-3 antigen) nor progeny virus is produced. Maximum levels of HHV-6 antigen (IEA/ex3 antigen)-positive cells (36.4%) were observed 48 hr after viral infection. Cocultivation of HHV-6-infected cord blood mononuclear cells with A431 cells was necessary for the establishment of a sufficient level of viral infection. Cell-to-cell contact between the infected cord blood mononuclear cells and A431 cells was crucial for increasing infection efficiency. To determine the biological effect of HHV-6 infection, flow cytometric analysis was carried out in HHV-6- and mock-infected A431 cells. Although no alteration was observed in VCAM-1 and ELAM-1 expression, that of HLA-ABC, HLA-DR, and ICAM-1 was upregulated after infection with HHV-6.

• Authors: Tetsushi Yoshikawa, Fumi Goshima, Shiho Akimoto, Takao Ozaki, Takuya Iwasaki, Takeshi Kurata, Yoshizo Asano, Yukihiro Nishiyama
• Journal: Journal of medical virology (J Med Virol) Vol. 71 Issue 1 Pg. 62-8 (Sep 2003) ISSN: 0146-6615 [Print] United States
• PMID: 12858410 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2003 Wiley-Liss, Inc.

Chemical References

• HLA Antigens
• Intercellular Adhesion Molecule-1

Topics

• Cell Line
• Epidermis (metabolism, pathology, virology)
• Flow Cytometry
• Gene Expression Regulation
• HLA Antigens (metabolism)
• Herpesvirus 6, Human (physiology)
• Humans
• Intercellular Adhesion Molecule-1 (metabolism)