Development of a new less-sensitive enzyme immunoassay for detection of early HIV-1 infection.

Abstract

The sensitive/less-sensitive (S/LS) enzyme immunoassay (EIA) testing strategy for discriminating "early" from "longstanding" HIV infection has been widely applied for detecting recent seroconverters and estimating HIV incidence rates. The originally developed assay (3A11-LS EIA; Abbott Laboratories, Abbott Park, IL) involved performance of LS EIAs using a bead-based assay that required specialized equipment and reagents of limited availability. In contrast, 96-microwell-based EIAs are more universally applied for HIV serodiagnosis throughout the world. The authors report development and preliminary validation of an LS protocol using an EIA in a 96-well format: the Vironostika HIV-1 MicroElisa System (Vironostika-LS EIA; Bio Merieux, Raleigh, NC). The results with samples from recent HIV-1 seroconverters, persons with longstanding HIV-1 asymptomatic infection, patients on highly active antiretroviral therapy, and AIDS patients show a high degree of correlation between the Vironostika-LS EIA and 3A11-LS EIA. The authors also demonstrate that the Abbott 3A11-LS EIA and Vironostika-LS EIA performed comparably on HIV-1-positive samples from persons infected with non-B HIV-1 subtypes. These results support the potential use of the Vironostika-LS EIA for detection of recent HIV-1 infections for incidence projections and for other epidemiologic, clinical, and molecular surveillance applications.

• Authors: Bhupat D Rawal, Azucena Degula, Ludmila Lebedeva, Robert S Janssen, Frederick M Hecht, Haynes W Sheppard, Michael P Busch
• Journal: Journal of acquired immune deficiency syndromes (1999) (J Acquir Immune Defic Syndr) Vol. 33 Issue 3 Pg. 349-55 (Jul 01 2003) ISSN: 1525-4135 [Print] United States
• PMID: 12843746 (Publication Type: Evaluation Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• HIV Antibodies

Topics

• Acquired Immunodeficiency Syndrome (diagnosis, immunology, virology)
• Female
• HIV Antibodies (blood)
• HIV Infections (diagnosis, immunology, virology)
• HIV-1 (immunology, isolation & purification)
• Humans
• Immunoenzyme Techniques (methods)
• Male
• Sensitivity and Specificity