Tissue macroarray: a simple and cost-effective method for high-throughput studies.

Abstract

The tissue microarray technique is a powerful technique that can examine many clinical specimens in a single slide. However, this technique requires a specialized instrument for the construction of tissue microarray block. The authors describe an alternative tissue macroarray technique for the simultaneous analysis of multiple clinical specimens on a single slide. As an example of this method, the authors assembled 12 tumor samples from 12 different paraffin blocks on a glass slide and then immunostained with hMLH1 antibody. This simple method does not need specialized equipment and should be an alternative efficient approach for large-scale clinical specimens.

• Authors: Liang Wang, Michael T Deavers, Anais Malpica, Elvio G Silva, Jinsong Liu
• Journal: Applied immunohistochemistry & molecular morphology : AIMM (Appl Immunohistochem Mol Morphol) Vol. 11 Issue 2 Pg. 174-6 (Jun 2003) ISSN: 1541-2016 [Print] United States
• PMID: 12778004 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal

Topics

• Antibodies, Monoclonal
• Female
• Humans
• Immunohistochemistry (economics, methods)
• Microtomy
• Ovarian Neoplasms (pathology)
• Paraffin Embedding
• Staining and Labeling