Targeting of post-ischemic cerebral endothelium in rat by liposomes bearing polyethylene glycol-coupled transferrin.

Abstract

To achieve an efficient delivery targeting to post-ischemic cerebral vascular endothelium, PEG-liposome conjugated with transferrin (Tf) (Tf-PEG-liposome) was intravenously administered to the rats after 90 min of transient middle cerebral occlusion. The expression of Tf receptor (TfR) in the cerebral endothelium increased with a peak at 1 day after the reperfusion and returned to the control level by 6 days. The Tf-PEG fluorescence was marginally detectable in sham control brain, but remarkably increased with a peak at 2 days, showing about 70% of TfR positive vascular endothelium double-labeled with Tf-PEG. These results indicate that the Tf-PEG-liposome could be utilized as an efficient drug delivery tool to the brain after stroke.

• Authors: N Omori, K Maruyama, G Jin, F Li, S J Wang, Y Hamakawa, K Sato, I Nagano, M Shoji, K Abe
• Journal: Neurological research (Neurol Res) Vol. 25 Issue 3 Pg. 275-9 (Apr 2003) ISSN: 0161-6412 [Print] England
• PMID: 12739237 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Liposomes
• Receptors, Transferrin
• Transferrin
• Polyethylene Glycols

Topics

• Animals
• Blood-Brain Barrier
• Brain Ischemia (drug therapy, metabolism)
• Endothelium, Vascular (metabolism)
• Infarction, Middle Cerebral Artery (metabolism)
• Liposomes
• Male
• Polyethylene Glycols (administration & dosage)
• Rats
• Rats, Wistar
• Receptors, Transferrin (biosynthesis)
• Telencephalon (blood supply)
• Transferrin (administration & dosage, metabolism, pharmacokinetics)