LB30057, an orally effective direct thrombin inhibitor, prevents arterial and venous thrombosis in rats and dogs.

Abstract

The anti-thrombotic effects of LB30057, a direct thrombin inhibitor, were evaluated with in vivo rat and dog thrombosis models. In rats, 1 mg/kg of LB30057 inhibited half of the clot formations in the inferior vena cava at 5 minutes after intravenous application. When measured at 2 hours after oral application, 100 mg/kg prevented approximately half of the clot formations in the inferior vena cava and 50 mg/kg prolonged the mean occlusion time from 15.6 +/- 1.3 minutes to 47.2 +/- 8.3 minutes in the carotid artery. In dogs, the formation of thrombus in the jugular vein was reduced to half at a dose range of 20-30 mg/kg at 6 hours after oral application. In addition, the LB30057 dosage required to reduce venous clot formation by approximately 80-90% in dogs was only about 10% of that required for the same reduction in rats. This is probably due to the variation in its time-dependent blood concentration profiles in each species; for example, the plasma half-life of LB71350 in dogs was longer than that in rats (153.0 +/- 3.0 vs. 129.7 +/- 12.7 min at 30 mg/kg, i.v., respectively). AUC, T(max), C(max), and BA in dogs were 59, 8.9, 9.17, and 13.3 times higher than those in rats at oral 30 mg/kg, respectively. Taken together, these results suggest that LB30057 administered orally is effective in the prevention of arterial and venous thrombosis in rats and dogs. It therefore represents a good lead compound for investigations to discover a new, orally available, therapeutic agent for treating thrombotic diseases.

• Authors: Hee Dong Park, Hee Jin Kim, Yeong Soo Oh, In Chull Kim, Yong Zu Kim, Hyun Chul Koh, In Chul Shin, Yong Hee Lee, Chang Ho Lee
• Journal: Archives of pharmacal research (Arch Pharm Res) Vol. 26 Issue 3 Pg. 224-31 (Mar 2003) ISSN: 0253-6269 [Print] Korea (South)
• PMID: 12723936 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzamides
• Fibrinolytic Agents
• LB 30057

Topics

• Administration, Oral
• Animals
• Benzamides (administration & dosage, blood)
• Carotid Artery Thrombosis (metabolism, prevention & control)
• Dogs
• Fibrinolytic Agents (administration & dosage)
• Male
• Rats
• Rats, Sprague-Dawley
• Venous Thrombosis (metabolism, prevention & control)