[The growth inhibition of colorectal adenoma cells by sulindac and its mechanisms].

Abstract

OBJECTIVE:

This study was conducted to assess the growth inhibition of colorectal adenoma cells by sulindac and identify the possible mechanisms.

METHODS:

The colorectal adenoma cells from human sporadic adenomatous polyps were cultured, and then treated with sulindac. The cell viability was examined by MTT colorimetric assay; the S-phase fraction and the percentage of apoptosis were measured by flow cytometry.

RESULTS:

Following sulindac treatment at different concentrations for 24, 48 and 72 hours, reduction of the cell viability was time- and dose-dependent. After 48-hour-treatment, S-phase fraction was decreased and the percentage of apoptosis was increased; both indexes of all groups except 0.3 mmol/L group were different from those of controls (P < 0.05).

CONCLUSION:

These data suggested that sulindac could inhibit the growth of the colorectal adenoma cells, and its mechanisms might be related to suppressing proliferation and inducing apoptosis.

• Authors: Libin Huang, Qin Ouyang, Dapeng Wei, Xiaofang Liu, Renwei Hu
• Journal: Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao (Hua Xi Yi Ke Da Xue Xue Bao) Vol. 33 Issue 1 Pg. 101-3 (Jan 2002) ISSN: 0257-7712 [Print] China
• PMID: 12599442 (Publication Type: English Abstract, Journal Article)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Sulindac
• Prostaglandin-Endoperoxide Synthases

Topics

• Adenoma (pathology)
• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Apoptosis (drug effects)
• Cell Division (drug effects)
• Colonic Neoplasms (pathology)
• Female
• Humans
• Middle Aged
• Prostaglandin-Endoperoxide Synthases (metabolism)
• Sulindac (pharmacology)
• Tumor Cells, Cultured