Abstract |
Starting with an extract derived from the root of Cynanchum paniculatum Kitagawa (Asclepiadaceae) that was active in the process of inhibiting the growth of human cancer cells in culture, a phenanthroindolizidine alkaloid antofine was isolated and identified as an active principle (IC50 = 7.0 +/- 0.2 ng/ml for A549, human lung cancer cells; IC50 = 8.6 +/- 0.3 ng/ml for Col2, human colon cancer cells). Prompted by the high potency of cancer cell growth inhibition, additional action mechanism studies were performed with antofine. Utilizing cultured Col2 cells as a model, antofine induced arrest in the G2/M phase of the cell cycle after 48 h of incubation. With wash-out experiment, colony formation was also inhibited in a dose-dependent manner. These data suggest the potential of antofine to serve as a cancer chemotherapeutic agent by virtue of arresting the cell cycle.
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Authors | Sang Kook Lee, Kyung-Ae Nam, Yoen-Hoi Heo |
Journal | Planta medica
(Planta Med)
Vol. 69
Issue 1
Pg. 21-5
(Jan 2003)
ISSN: 0032-0943 [Print] Germany |
PMID | 12567274
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents, Phytogenic
- Indoles
- Phenanthrolines
- antofine
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Topics |
- Antineoplastic Agents, Phytogenic
(pharmacology)
- Cell Cycle
(drug effects)
- Cynanchum
(chemistry)
- Drug Screening Assays, Antitumor
- Humans
- Indoles
- Phenanthrolines
(isolation & purification, pharmacology)
- Tumor Cells, Cultured
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