Presence of pannus-like tissue on osteoarthritic cartilage and its histological character.

Abstract

OBJECTIVE:

To investigate and characterize pannus-like tissue which is often present on osteoarthritic articular cartilage.

DESIGN:

Cartilage specimens from 15 knee and five hip joints of patients with osteoarthritis (OA) undergoing arthroplasty were stained for HE and Safranin-O. They were also immunostained by antitype I collagen, type II collagen, CD68, IL-1beta and MMP3 antibodies.

RESULTS:

Ninety percent of joints have pannus-like tissue on the articular surface, preferentially in a marginal area. The articular cartilage was divided into three regions according to the location: the marginal zone, the intermediate zone and the paraeburnated zone. Pannuslike tissue in OA knee joint occurred 45.9%, 27.5% and 11.1% of the surface of each region respectively. Histologically, pannus-like tissue could be classified into the vascular type and the fibrous type. Extracellular matrix of both types of tissues were negative for Safranin-O and type II collagen, but positive for type I collagen. IL-1beta and MMP3 expressing cells are predominant in pannus-like tissue, whereas CD68 positive cells were infiltrated in only a few samples. Vascular type tissue kept continuity with bone marrow suggesting mesenchymal origin.

CONCLUSION:

Pannus-like tissue exists in advanced OA cartilage, preferentially in the marginal zone. It expressed IL-1beta and MMP3, which strongly suggests that it contributes to cartilage degradation.

• Authors: A Shibakawa, H Aoki, K Masuko-Hongo, T Kato, M Tanaka, K Nishioka, H Nakamura
• Journal: Osteoarthritis and cartilage (Osteoarthritis Cartilage) Vol. 11 Issue 2 Pg. 133-40 (Feb 2003) ISSN: 1063-4584 [Print] England
• PMID: 12554129 (Publication Type: Journal Article)
• Copyright: Copyright 2003 Published by Elsevier Science Ltd on behalf of OsteoArthritis Research Society International.

Chemical References

• Antigens, CD
• Antigens, Differentiation, Myelomonocytic
• CD68 antigen, human
• Interleukin-1
• Collagen
• Matrix Metalloproteinase 3

Topics

• Aged
• Antigens, CD (analysis)
• Antigens, Differentiation, Myelomonocytic (analysis)
• Cartilage, Articular (pathology)
• Collagen (analysis)
• Extracellular Matrix (pathology)
• Female
• Hip Joint (pathology)
• Humans
• Immunohistochemistry (methods)
• Interleukin-1 (analysis)
• Knee Joint (pathology)
• Matrix Metalloproteinase 3 (analysis)
• Middle Aged
• Osteoarthritis (pathology)
• Osteoarthritis, Hip (pathology)
• Osteoarthritis, Knee (pathology)