[Cloning, sequencing of beagle E-selectin genomic DNA and comparison of it with other species].

Abstract

OBJECTIVE:

Selectins are a group of members of Cell Adhesion Molecular family; they play very important roles in inflammation and neovascularization. The aim of this study is to clone, sequence Beagle E-selectin genomic DNA and make homogeneous analyses to compare it with those of other animals.

METHODS:

Twelve fragments of genomic DNA of Beagle E-selectin were obtained by PCR, and the 12 fragments were cloned in T-vector. At last, the genomic DNA sequences of Beagle E-selectin were obtained through gene sequencing, assembling and base calling, and the gene structure was analyzed.

RESULTS:

The sequence of Beagle E-selectin genomic DNA that contained the whole open reading box was acgured. The results of homogeneous analyses showed a great extent of E-selectin gene homology among human, porcine and dog.

CONCLUSION:

The demonstration of a large extent of the gene homology has substantiated in this connection the important roles of E-selectin in transplantation immunity and neoplasm development.

• Authors: L Zheng, Y Shi, L Dong, G Zhang, H Wu, Y Li, S Cheng
• Journal: Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao (Hua Xi Yi Ke Da Xue Xue Bao) Vol. 32 Issue 3 Pg. 325-9 (Sep 2001) ISSN: 0257-7712 [Print] China
• PMID: 12536553 (Publication Type: Comparative Study, English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• E-Selectin

Topics

• Animals
• Cloning, Molecular
• Dogs
• E-Selectin (genetics, physiology)
• Genome
• Humans
• Polymerase Chain Reaction (methods)
• Sequence Analysis, DNA
• Sequence Homology
• Swine