Arginase activity is inhibited by L-NAME, both in vitro and in vivo.

Abstract

The present study investigated the ability of the arginine analog L-NAME (N(omega)-Nitro-L-arginine methyl ester) to modulate the activity of arginase. L-NAME inhibited the activity of arginase in lysates from rat colon cancer cells and liver. It also inhibited the arginase activity of tumor cells in culture. Furthermore, in vivo treatment of rats with L-NAME inhibited arginase activity in tumor nodules and liver, and the effect persisted after treatment ceased. The effect of L-NAME on arginase requires consideration when it is used in vivo in animal models with the aim of inhibiting endothelial NO-synthase, another enzyme using arginine as substrate.

• Authors: Danièle Reisser, Nathalie Onier-Cherix, Jean-François Jeannin
• Journal: Journal of enzyme inhibition and medicinal chemistry (J Enzyme Inhib Med Chem) Vol. 17 Issue 4 Pg. 267-70 (Aug 2002) ISSN: 1475-6366 [Print] England
• PMID: 12530480 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Enzyme Inhibitors
• Neoplasm Proteins
• Arginase
• NG-Nitroarginine Methyl Ester

Topics

• Animals
• Arginase (antagonists & inhibitors)
• Colonic Neoplasms (drug therapy, enzymology, pathology)
• Dose-Response Relationship, Drug
• Enzyme Inhibitors (administration & dosage, pharmacology)
• Liver (enzymology)
• NG-Nitroarginine Methyl Ester (administration & dosage, pharmacology)
• Neoplasm Proteins (drug effects)
• Rats
• Treatment Outcome
• Tumor Cells, Cultured