Abstract | OBJECTIVE: METHODS: Recombinant adenoviral vectors, AdVEGF-tk and AdVEGF-GFP, were constructed with HSV-tk or GFP under the control of VEGF promoter through AdEasy system. Then GFP expression in hepatoma cell line HepG2 and normal liver cell line L02 transfected with AdVEGF-GFP were observed under fluorescence microscope, and the sensitivity to GCV of the AdVEGF-tk-transfected cells under normoxia or hypoxia condition were monitored by MTT method. RESULTS: GFP expression actuated by VEGF promoter was detected in sporadic L02 cells, but in almost all HepG2 cells after transfected with AdVEGF-GFP. With GCV at 10 micro g/ml and MOI at 100, L02 cells were insensitive to GCV under oxic condition, but more than 70% L02 cells were killed under hypoxic condition. Moreover, HepG2 cells infected with AdVEGF-tk showed the increased GCV sensitivity under hypoxia (over 80% killed) as compared with normoxia (over 60% killed) conditions. CONCLUSION:
Hypoxia enhances the GCV sensitivity of human hepatocellular tumor cells infected with recombinant AdVEGF-tk under the control of VEGF promoter.
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Authors | Menglong Wang, Zhengfeng Yin, Zongdi Wu, Shuai Wang, Haihua Qian, Xiaoyan Kang, Mengchao Wu |
Journal | Zhonghua zhong liu za zhi [Chinese journal of oncology]
(Zhonghua Zhong Liu Za Zhi)
Vol. 24
Issue 5
Pg. 455-7
(Sep 2002)
ISSN: 0253-3766 [Print] China |
PMID | 12485497
(Publication Type: Journal Article)
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Chemical References |
- Endothelial Growth Factors
- Intercellular Signaling Peptides and Proteins
- Lymphokines
- Vascular Endothelial Growth Factor A
- Vascular Endothelial Growth Factors
- Thymidine Kinase
- Oxygen
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Topics |
- Adenoviridae
(genetics)
- Carcinoma, Hepatocellular
(pathology)
- Endothelial Growth Factors
(genetics)
- Gene Expression
(drug effects)
- Gene Transfer Techniques
- Genetic Vectors
(genetics)
- Humans
- Hypoxia
- Intercellular Signaling Peptides and Proteins
(genetics)
- Liver Neoplasms
(pathology)
- Lymphokines
(genetics)
- Oxygen
(pharmacology)
- Promoter Regions, Genetic
(physiology)
- Simplexvirus
(enzymology)
- Thymidine Kinase
(genetics, metabolism)
- Tumor Cells, Cultured
- Vascular Endothelial Growth Factor A
- Vascular Endothelial Growth Factors
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