Abstract | BACKGROUND: METHODS:
Glucoamylase and other disaccharidase specific activities were assayed from duodenal biopsy specimens in 44 children aged 0.5-18 years (mean, 10 +/- 5 years) undergoing endoscopy to evaluate dyspeptic symptoms. All subjects had normal duodenal histology. Intestinal organ culture was used to evaluate synthesis and processing of maltase-glucoamylase. Sequencing of the maltase-glucoamylase coding region was performed in subjects with low activity or variation of isoform in organ culture. RESULTS: CONCLUSION:
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Authors | Wikrom Karnsakul, Ursula Luginbuehl, Dagmar Hahn, Erwin Sterchi, Stephen Avery, Partha Sen, Dallas Swallow, Buford Nichols |
Journal | Journal of pediatric gastroenterology and nutrition
(J Pediatr Gastroenterol Nutr)
Vol. 35
Issue 4
Pg. 551-6
(Oct 2002)
ISSN: 0277-2116 [Print] United States |
PMID | 12394383
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- DNA, Complementary
- Isoenzymes
- Disaccharidases
- Glucosidases
- Lactase
- alpha-Glucosidases
- beta-Galactosidase
- Sucrase
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Topics |
- Biopsy
- DNA, Complementary
(analysis)
- Disaccharidases
(deficiency, metabolism)
- Duodenum
(enzymology, pathology)
- Dyspepsia
(enzymology)
- Electrophoresis, Polyacrylamide Gel
- Female
- Glucosidases
(deficiency, metabolism)
- Humans
- Infant
- Intestinal Mucosa
(enzymology, pathology)
- Isoenzymes
- Lactase
- Male
- Reverse Transcriptase Polymerase Chain Reaction
- Sucrase
(deficiency, metabolism)
- alpha-Glucosidases
(deficiency, metabolism)
- beta-Galactosidase
(deficiency, metabolism)
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