Angiogenesis inhibitor TNP-470 suppresses growth of peritoneal disseminating foci of human colon cancer line Lovo.

Abstract

AIM:

To study the effect of angiogenesis inhibitor TNP-470 on peritoneal dissemination of colon cancer in nude mice.

METHODS:

The MTT assay was used to evaluate the inhibitory effect of TNP-470 on human colon cancer cell line Lovo. Lovo cells were injected into the peritoneal cavity of BABL/C nu/nu mice and the models of peritoneal dissemination were developed. Thirty nude mice were randomly divided into control and TNP-470-treated group. In TNP-470-treated group, TNP-470 was injected subcutaneously every other day from day 1 until sacrifice or death (30 mg x kg(-1)). The control group received a sham injection of the same volume saline solution.

RESULTS:

In vitro, TNP-470 inhibited the growth of Lovo cells, with its IC50 at 2.14 X 10(2) microg x L(-1). In vitro, TNP-470 demonstrated growth inhibition of tumors. Mice body weight and abdominal circumferences were significantly different between TNP-470-treated group (24.5+/-3.2 g, 7.0+/-1.1 cm) and control group (29.5+/-2.1 g, 10.3+/-1.5 cm), P=0.005 and P=0.001. The number of disseminated foci was significantly different between the control group (92.1+/-20.6) and the TNP-470-treated group (40.3+/-12.3), P<0.001. The maximal size of foci was significantly smaller in TNP-470-treated group (3.3+/-0.7 mm) than that of control (7.3+/-2.3 mm), P=0.004. Mean survival time was significantly longer in TNP-470-treated group(98.00+/-12.06 d) than that in control group (41.86+/-9.51 d), P<0.001.

CONCLUSION:

Angiogenesis inhibitor TNP-470 might be effective in treating peritoneal dissemination of colon cancer and improve the survival rate of nude mice.

• Authors: Ying-Fang Fan, Zong-Hai Huang
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 8 Issue 5 Pg. 853-6 (Oct 2002) ISSN: 1007-9327 [Print] United States
• PMID: 12378629 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Angiogenesis Inhibitors
• Cyclohexanes
• Sesquiterpenes
• O-(Chloroacetylcarbamoyl)fumagillol

Topics

• Adenocarcinoma (drug therapy, mortality)
• Angiogenesis Inhibitors (pharmacology)
• Animals
• Cell Division (drug effects)
• Colonic Neoplasms (drug therapy, mortality)
• Cyclohexanes
• Female
• Humans
• In Vitro Techniques
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• O-(Chloroacetylcarbamoyl)fumagillol
• Peritoneum (pathology)
• Sesquiterpenes (pharmacology)
• Tumor Cells, Cultured