COP9 signalosome subunits 4 and 5 regulate multiple pleiotropic pathways in Drosophila melanogaster.

Abstract	The COP9 signalosome (CSN) is an essential eight-subunit repressor of light-regulated development in Arabidopsis. This complex has also been identified in animals, though its developmental role remains obscure. CSN subunits have been implicated in various cellular processes, suggesting a possible role for the CSN as an integrator of multiple signaling pathways. In order to elucidate the function of the CSN in animals, a Drosophila model system has previously been established. Gel-filtration analysis with antibodies against CSN subunits 4, 5 and 7 revealed that these proteins act as a complex in Drosophila that is similar in size to the plant and mammalian complexes. Null mutations in either one of two subunits, CSN4 or CSN5, are larval lethal. Successful embryogenesis appears to be a consequence of maternal contribution of the complex. Biochemical analysis indicates that the different subunits are found in both CSN-dependent and CSN-independent forms, and that these forms are differentially affected by the mutations. Phenotypic characterization of these two mutants indicates that they show both shared and unique phenotypes, which suggest specific roles for each subunit. Both mutants have defective oocyte and embryo patterning, and defects in response to DNA damage, while csn5 mutants develop melanotic tumors and csn4 mutants have phenotypes reminiscent of defects in ecdysone signaling.
Authors	Efrat Oron, Mattias Mannervik, Sigal Rencus, Orit Harari-Steinberg, Shira Neuman-Silberberg, Daniel Segal, Daniel A Chamovitz
Journal	Development (Cambridge, England) (Development) Vol. 129 Issue 19 Pg. 4399-409 (Oct 2002) ISSN: 0950-1991 [Print] England
PMID	12223399 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Drosophila Proteins Multiprotein Complexes Proteins Repressor Proteins Methyl Methanesulfonate Peptide Hydrolases COP9 Signalosome Complex
Topics	Animals Body Patterning COP9 Signalosome Complex Cell Polarity Drosophila Proteins (genetics, metabolism) Drosophila melanogaster (embryology, genetics, metabolism) Larva Methyl Methanesulfonate (pharmacology) Multiprotein Complexes Mutagenesis Oocytes Oogenesis Peptide Hydrolases Phenotype Proteins (genetics, metabolism) Repressor Proteins (genetics, metabolism) Signal Transduction

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