Human immunodeficiency virus type 1 Vif binds the viral protease by interaction with its N-terminal region.

Abstract

The vif gene, one of the six auxiliary genes of human immunodeficiency virus (HIV), is essential for virus propagation in peripheral blood lymphocytes and macrophages and in certain T-cell lines. Previously, it was demonstrated that Vif inhibits the autoprocessing of truncated HIV type 1 (HIV-1) Gag-Pol polyproteins expressed in bacterial cells, as well as the protease-mediated cleavage of synthetic peptides in vitro. Peptides derived from the aa 78-98 region in the Vif molecule specifically inhibit and bind the HIV-1 protease in vitro and arrest the production of infectious viruses in HIV-1-infected cells. This study demonstrates that (i) purified recombinant Vif protein and HIV-1 but not avian sarcoma leukaemia virus protease specifically bind each other and (ii) the interaction between these two proteins takes place at the N terminus of the protease (aa 1-9) and the central part of Vif (aa 78-98). The data presented in this report suggest a model in which Vif interacts with the dimerization sites of the viral protease.

• Authors: Lea Baraz, Marina Hutoran, Immanuel Blumenzweig, Mark Katzenellenbogen, Assaf Friedler, Chaim Gilon, Michael Steinitz, Moshe Kotler
• Journal: The Journal of general virology (J Gen Virol) Vol. 83 Issue Pt 9 Pg. 2225-2230 (Sep 2002) ISSN: 0022-1317 [Print] England
• PMID: 12185277 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Gene Products, vif
• HIV Protease Inhibitors
• Peptide Fragments
• Recombinant Proteins
• vif Gene Products, Human Immunodeficiency Virus
• HIV Protease

Topics

• Binding Sites
• Dimerization
• Dose-Response Relationship, Drug
• Gene Products, vif (biosynthesis, genetics, metabolism)
• HIV Protease (chemistry, genetics, metabolism)
• HIV Protease Inhibitors (chemical synthesis, pharmacology)
• HIV-1 (metabolism)
• Peptide Fragments (chemical synthesis, pharmacology)
• Protein Binding
• Recombinant Proteins (metabolism)
• Virus Replication
• vif Gene Products, Human Immunodeficiency Virus