[Role of vascular endothelial growth factor overexpression in ovarian tumor invasion and mechanism].

Abstract

OBJECTIVE:

To study the role of vascular endothelial growth factor (VEGF) overexpression in ovarian tumor metastasis and associated mechanism.

METHODS:

The VEGF cDNA was transfected into ovarian tumor cell lines CAOV3 and COC1. Transfected and nontransfected cells were screened for VEGF, gelatinase A (MMP-2) mRNA and protein by reverse transcription polymerase chain reaction (RT-PCR), Western blot and substrate zymography, respectively. The invasive ability of two ovarian tumor cell lines was analysed with Boyden chamber invasion assay before and after VEGF cDNA transfection.

RESULTS:

The expression of VEGF, MMP-2 mRNA and protein were increased (P < 0.05) after VEGF cDNA transfection, detected by RT-PCR, Western blot and substrate zymography. After VEGF cDNA transfection, the mean invasion percentage of two ovarian tumor cells [CAOV3: (42.5 +/- 4.1)%; COC1: (26.8 +/- 2.4)%] were higher than that before transfection [CAOV3: (24.7 +/- 1.9)%; COC1: (8.6 +/- 1.1)%, P < 0.05].

CONCLUSION:

The expression of VEGF correlates to the in vitro invasion of ovarian tumor cell and induction of MMP-2 by VEGF is a key component of VEGF-induced ovarian tumor cell invasion.

• Authors: Yunping Lu, Ali Zhang, Shixuan Wang, Jin Li, Changyu Wang, Ding Ma
• Journal: Zhonghua fu chan ke za zhi (Zhonghua Fu Chan Ke Za Zhi) Vol. 37 Issue 5 Pg. 294-7 (May 2002) ISSN: 0529-567X [Print] China
• PMID: 12133405 (Publication Type: English Abstract, Journal Article)

Chemical References

• DNA, Complementary
• Endothelial Growth Factors
• Lymphokines
• RNA, Messenger
• Vascular Endothelial Growth Factor A
• Vascular Endothelial Growth Factors
• Matrix Metalloproteinase 2

Topics

• DNA, Complementary (genetics)
• Endothelial Growth Factors (genetics, physiology)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Lymphokines (genetics, physiology)
• Matrix Metalloproteinase 2 (genetics, metabolism)
• Neoplasm Invasiveness
• Ovarian Neoplasms (genetics, pathology)
• RNA, Messenger (genetics, metabolism)
• Transfection
• Tumor Cells, Cultured
• Vascular Endothelial Growth Factor A
• Vascular Endothelial Growth Factors