Fuc(alpha1-->3)GalNAc-: the major antigenic motif of Schistosoma mansoni glycolipids implicated in infection sera and keyhole-limpet haemocyanin cross-reactivity.

Abstract

The aim of the present study was the characterization of the dominant epitope present on Schistosoma mansoni glycolipids, which causes cross-reactivity of S. mansoni and S. haematobium infection sera with keyhole-limpet haemocyanin (KLH). To this end, the monoclonal antibody M2D3H was chosen for its similar behaviour in high-performance TLC immunostaining and inhibition-ELISA to infection sera. Individual, structurally defined oligosaccharides derived from S. mansoni egg glycolipids were tested for their binding to this monoclonal antibody by immunoaffinity chromatography. A terminal fucose residue linked in the (alpha1-->3) position to N-acetylgalactosamine was found to be the common structural determinant of the four oligosaccharides binding to M2D3H. The Fuc(alpha1-->3)GalNAc-motif also appeared to be the basis for the cross-reactivity with KLH, a phenomenon used in the serodiagnosis of S. mansoni, S. haematobium and S. japonicum infections.

• Authors: Sven R Kantelhardt, Manfred Wuhrer, Roger D Dennis, Michael J Doenhoff, Quentin Bickle, Rudolf Geyer
• Journal: The Biochemical journal (Biochem J) Vol. 366 Issue Pt 1 Pg. 217-23 (Aug 15 2002) ISSN: 0264-6021 [Print] England
• PMID: 11996672 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens
• Glycolipids
• Oligosaccharides
• Polysaccharides
• fucosyl alpha 1,3 N-acetylglucosamine
• Acetylglucosamine

Topics

• Acetylglucosamine (analogs & derivatives, chemistry)
• Amino Acid Motifs
• Animals
• Antibody Specificity
• Antigens (chemistry)
• Chromatography, Thin Layer
• Enzyme-Linked Immunosorbent Assay
• Glycolipids (chemistry)
• Oligosaccharides (chemistry)
• Polysaccharides (chemistry)
• Protein Structure, Tertiary
• Schistosoma mansoni (metabolism)
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• Time Factors