The expression pattern of the ITIM-bearing lectin CLECSF6 in neutrophils suggests a key role in the control of inflammation.

Abstract	In our study of the modulation of the expression of inflammation-related genes in neutrophils, we have found a gene called CLECSF6 (C-type lectin superfamily 6). CLECSF6 expresses two mRNA species at low levels in resting neutrophils. Here, we describe for the first time the sequence of the short mRNA version. It lacks amino acids that are likely to affect the functionality of its protein product. GM-CSF, IL-3, IL-4, and IL-13 caused an accumulation of the short CLECSF6 mRNA in neutrophils. The surface expression of the CLECSF6 protein was reduced by TNF-alpha, IL-1alpha, LPS, and Matrigel. CLECSF6 bears the immunoreceptor tyrosine-based inhibition motif (ITIM) involved in signal transduction resulting in the inhibition of leukocyte activation. We propose that some neutrophil activators modulate the expression of CLECSF6 at the mRNA (GM-CSF, IL-3, IL-4, and IL-13) or protein (TNF-alpha, IL-1alpha, LPS, and Matrigel) levels in ways that block ITIM-based transduction of anti-inflammatory signals and therefore promote inflammation.
Authors	Manon Richard, Patricia Veilleux, Michèle Rouleau, Robert Paquin, André D Beaulieu
Journal	Journal of leukocyte biology (J Leukoc Biol) Vol. 71 Issue 5 Pg. 871-80 (May 2002) ISSN: 0741-5400 [Print] England
PMID	11994513 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	CLEC4A protein, human Interleukins Lectins Lectins, C-Type Membrane Glycoproteins Protein Isoforms RNA, Messenger Receptors, Immunologic Granulocyte-Macrophage Colony-Stimulating Factor
Topics	Amino Acid Motifs Amino Acid Sequence Cell Differentiation Down-Regulation Gene Expression Regulation Granulocyte-Macrophage Colony-Stimulating Factor (pharmacology) HL-60 Cells Humans Inflammation (immunology) Interleukins (pharmacology) Lectins (biosynthesis, chemistry, genetics, physiology) Lectins, C-Type Membrane Glycoproteins Molecular Sequence Data Myeloid Cells (immunology) Neutrophils (drug effects, immunology) Open Reading Frames Polymerase Chain Reaction Protein Isoforms (biosynthesis, genetics) RNA, Messenger (analysis) Receptors, Immunologic Transcription, Genetic (drug effects)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!