Antisense of ATP synthase subunit e inhibits the growth of human hepatocellular carcinoma cells.

Abstract

Differentially expressed genes between normal and hepatocellular carcinoma tissues were investigated using differential display. We identified a cDNA fragment that was overexpressed in cancer tissue. Homology analysis showed that the sequence was identical to human ATP synthase subunit e (hAS-e). Moreover, Northern blot analysis demonstrated that hAS-e was overexpressed in 10 of 11 (91%) specimens of hepatocellular carcinoma compared with corresponding normal tissues. We introduced antisense hAS-e into a human hepatocellular carcinoma BEL-7404 cell and found that downregulation of the hAS-e led to cell growth inhibition. It was also found that the antisense transfection could decrease the serum-stimulated activation of mitogen-activated protein kinase (MAP kinase). Together, the results suggest that antisense of hAS-e can inhibit cell proliferation through the MAP kinase pathway. Our data indicate that hAS-e may become a new target in gene therapy.

• Authors: H Ying, Y Yu, Y Xu
• Journal: Oncology research (Oncol Res) Vol. 12 Issue 11-12 Pg. 485-90 ( 2001) ISSN: 0965-0407 [Print] United States
• PMID: 11939412 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Primers
• Oligodeoxyribonucleotides, Antisense
• Protein Subunits
• ATP5ME protein, human
• Mitochondrial Proton-Translocating ATPases

Topics

• Base Sequence
• Carcinoma, Hepatocellular
• Cell Division (drug effects)
• Cloning, Molecular
• DNA Primers
• Humans
• Liver Neoplasms
• Mitochondrial Proton-Translocating ATPases (genetics)
• Oligodeoxyribonucleotides, Antisense (pharmacology)
• Protein Subunits
• Tumor Cells, Cultured