Glutamate and
kainate-induced currents of primate
ganglion cells were studied using the whole-cell patch-clamp technique in a
retinal slice preparation. Antagonists and allosteric modulators of desensitization selective for either alpha-amino-3-hydroxy-5-methyl-4-isoazoleprionic
acid (
AMPA)- or
kainate-preferring receptors were used to determine the contributions of each type of receptor to excitatory responses. With synaptic transmission and
NMDA receptors blocked, the
AMPA-preferring receptor antagonist
GYKI 52466 (30 microM-100 microM) reversibly blocked most of the
glutamate-induced current in
ganglion cells.
GYKI 52466 also blocked the response in
ganglion cells to focally applied
kainate, suggesting that the current response to
kainate arises from activation of
AMPA-preferring receptors, and not
kainate-preferring receptors. Both
cyclothiazide (10 microM-100 microM) and the novel
drug 4-[2-(phenylsulfonylamino)ethylthio]-2,6-difluoro-phenoxyacetamide (PEPA, 20 microM-100 microM), which selectively enhance responses mediated by
AMPA-preferring receptors, enhanced
glutamate-induced responses of
ganglion cells. Since these drugs preferentially inhibit desensitization of the flip and flop splice variants, respectively, of
AMPA-preferring receptors, it is likely that both splice variants are present on these
ganglion cells.
Concanavalin A, which selectively suppresses the desensitization of
kainate-preferring receptors, had no effect on the
glutamate-induced responses of
ganglion cells. We conclude that the non-
NMDA component of the excitatory, glutamatergic input to primate
ganglion cells is mediated largely by
AMPA-preferring receptors, with little, if any,
kainate-preferring receptor-mediated response.