Arachidonate release and eicosanoid generation by group IIE phospholipase A(2).

Abstract

The heparin-binding group II subfamily of secretory phospholipase A(2)s (sPLA(2)s), such as sPLA(2)-IIA and -IID, augments stimulus-induced arachidonic acid (AA) release through the cellular heparan sulfate proteoglycan (HSPG)-dependent pathway when transfected into HEK293 cells. Here we show that the closest homolog, sPLA(2)-IIE, also promotes stimulus-induced AA release and prostaglandin (PG) production similar to those elicited by HSPG-dependent sPLA(2)s. Confocal laser microscopic analysis demonstrates the location of sPLA(2)-IIE in cytoplasmic punctate compartments. sPLA(2)-IIE also enhances leukotriene (LT) production and granule exocytosis by RBL-2H3 mastocytoma cells. Expression of sPLA(2)-IIE was highly upregulated in mice injected with lipopolysaccharide (LPS) and in mice with experimental atopic dermatitis. These observations suggest that this enzyme plays a role in the inflammatory process, as proposed for other group II subfamily sPLA(2)s.

• Authors: Makoto Murakami, Kumiko Yoshihara, Satoko Shimbara, Gerard Lambeau, Alan Singer, Michael H Gelb, Masatsugu Sawada, Naoki Inagaki, Hiroichi Nagai, Ichiro Kudo
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 292 Issue 3 Pg. 689-96 (Apr 05 2002) ISSN: 0006-291X [Print] United States
• PMID: 11922621 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anticoagulants
• Eicosanoids
• Isoenzymes
• Arachidonic Acid
• Heparin
• Phospholipases A
• Group II Phospholipases A2

Topics

• Animals
• Anticoagulants (pharmacology)
• Arachidonic Acid (metabolism)
• Cell Line
• Eicosanoids (metabolism)
• Gene Expression Regulation
• Group II Phospholipases A2
• Heparin (pharmacology)
• Humans
• Inflammation (physiopathology)
• Isoenzymes (metabolism)
• Mast Cells (metabolism)
• Mice
• Microscopy, Confocal
• Phospholipases A (genetics, metabolism)
• Protein Binding