Abstract | BACKGROUND: METHODS: Human colon cancer cells (Caco-2 and HT-29) were treated with deoxycholate or its two structural isomers, 3-beta-12-alpha-dihydroxy-5-beta-cholan-24-oic acid and 3-alpha-12-beta-dihydroxy-5-beta-cholan-24-oic acid. Proliferation was evaluated by cell counting, and apoptosis by estimating percentage cell survival and assessment of nuclear morphology. RESULTS: Within the concentration range of up to 20 microM, deoxycholate stimulated growth of both human colon cancer cell lines. Its growth-promoting effect was abolished after inhibition of protein kinase C. At concentrations above 100 microM, deoxycholate induced apoptosis in both cell lines. Epimers of deoxycholate were significantly less potent in stimulating growth. CONCLUSION:
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Authors | V Milovic, I C Teller, D Faust, W F Caspary, J Stein |
Journal | European journal of clinical investigation
(Eur J Clin Invest)
Vol. 32
Issue 1
Pg. 29-34
(Jan 2002)
ISSN: 0014-2972 [Print] England |
PMID | 11851724
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Bile Acids and Salts
- Detergents
- Deoxycholic Acid
- Protein Kinase C
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Topics |
- Apoptosis
(drug effects)
- Bile Acids and Salts
(pharmacology)
- Caco-2 Cells
(cytology, drug effects, enzymology)
- Cell Division
(drug effects)
- Colonic Neoplasms
(pathology)
- Deoxycholic Acid
(pharmacology)
- Detergents
(pharmacology)
- HT29 Cells
(cytology, drug effects, enzymology)
- Humans
- Intestinal Mucosa
(pathology)
- Protein Kinase C
(metabolism)
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