[Expression of transforming growth factor-beta 1 mRNA and osteopontin mRNA in UMR-106 cells under mechanical stimulation].

Abstract

OBJECTIVE:

To investigate the effects of the mechanical stretching on the expression of osteopontin (OPN) mRNA and transforming growth factor-beta 1 (TGF beta 1) mRNA in a rat osteosarcoma cell line (UMR-106), and to unveil the cellular mechanism of mechanical force-induced bone remodeling.

METHODS:

An apparatus was designed and fabricated by which force was loaded onto the cultured cells in vitro. Digoxin-labeled cDNA probes were used for in situ hybridization in combination with image analysis technique to relatively quantify the intensities of the hybridization signals.

RESULTS:

Various magnitudes and durations of the mechanical stretching did exert different influences on the intensities of the mRNAs' expression. Of all the combinations, the low tension/frequency group showed the most remarkable alteration of TGF-beta 1, as increased from 0.0899 to 0.1756 (P < 0.01). Some putative relations lay between the expressions of the OPN mRNA and the TGF-beta 1 mRNA.

CONCLUSION:

The mechanical stretching can inevitably influence the expression of OPN mRNA and TGF-beta 1 mRNA. The beneficial alteration obtained in using low tension/frequency action mode suggests and verifies the use of relatively constant and light force to move teeth in clinical orthodontics.

• Authors: D Liu, M Fu, S Li
• Journal: Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology (Zhonghua Kou Qiang Yi Xue Za Zhi) Vol. 35 Issue 1 Pg. 27-30 (Jan 2000) ISSN: 1002-0098 [Print] China
• PMID: 11831958 (Publication Type: English Abstract, Journal Article)

Chemical References

• RNA, Messenger
• Sialoglycoproteins
• Spp1 protein, rat
• Tgfb1 protein, rat
• Transforming Growth Factor beta
• Transforming Growth Factor beta1
• Osteopontin

Topics

• Animals
• Orthodontics, Corrective
• Osteoblasts (metabolism)
• Osteopontin
• Physical Stimulation
• RNA, Messenger (biosynthesis)
• Rats
• Sialoglycoproteins (biosynthesis, genetics)
• Transforming Growth Factor beta (biosynthesis, genetics)
• Transforming Growth Factor beta1
• Tumor Cells, Cultured