Abstract |
The influence of chemical stability on the antimetastatic ruthenium(III) compound imidazolium trans-imidazoletetrachlorodimethylsulphoxideruthenium(III) ( NAMI-A) in aqueous solution was studied both in vitro and in vivo. The loss of dimethyl-sulphoxide ( DMSO) ligand from the compound was tested by using a NAMI-A solution acidified with HCl at pH 3.0 and aged for 0, 4, 8 and 24 h prior to intraperitoneal (i.p.) injection into CBA mice bearing advanced MCa mammary carcinoma. The activity of NAMI-A on lung metastases showed no change even after the loss of DMSO ligand from up to 50% of the molecules. The reduction of NAMI-A did not modify the number of KB cells blocked in the S+G2M phases, independent of whether the reduction occurred outside the cells or after loading the cells with the compound prior to treatment with the reductants ( ascorbic acid, glutathione or cysteine). In vivo, the complete reduction of NAMI-A with equivalent amounts of ascorbic acid, glutathione or cysteine prior to administration to mice bearing advanced MCa mammary carcinoma was more active than NAMI-A alone. The data show that NAMI-A, although undergoing a series of chemical modifications, maintains its antimetastatic activity in a broad range of experimental conditions.
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Authors | G Sava, A Bergamo, S Zorzet, B Gava, C Casarsa, M Cocchietto, A Furlani, V Scarcia, B Serli, E Iengo, E Alessio, G Mestroni |
Journal | European journal of cancer (Oxford, England : 1990)
(Eur J Cancer)
Vol. 38
Issue 3
Pg. 427-35
(Feb 2002)
ISSN: 0959-8049 [Print] England |
PMID | 11818210
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Organometallic Compounds
- dimethylsulfoxideimidazoletetrachlororuthenate(III)
- Dimethyl Sulfoxide
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Topics |
- Animals
- Antineoplastic Agents
(chemistry, pharmacokinetics, therapeutic use)
- Cell Division
- Dimethyl Sulfoxide
(analogs & derivatives, chemistry, metabolism, pharmacokinetics, therapeutic use)
- Female
- Lung Neoplasms
(secondary)
- Mammary Neoplasms, Experimental
(drug therapy, metabolism, pathology)
- Mice
- Mice, Inbred CBA
- Neoplasm Transplantation
- Organometallic Compounds
(chemistry, pharmacokinetics, therapeutic use)
- S Phase
- Tumor Cells, Cultured
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