PBK/TOPK is a novel mitotic kinase which is upregulated in Burkitt's lymphoma and other highly proliferative malignant cells.
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| Abstract |
PBK/TOPK is a recently cloned serine/threonine kinase which is phosphorylated during mitosis. Earlier work indicated that this kinase is upregulated in a Burkitt's lymphoma cell line (GA-10). To determine whether PBK/TOPK is upregulated in other mitotically active neoplastic cell lines and tissues, Northern analysis was performed on a panel of malignant cell lines and on clinical samples from patients with leukemia or lymphoma. While PBK/TOPK mRNA was not detectable in normal peripheral blood cells and was weakly expressed in hyperplastic tonsillar B-cells, significantly higher levels of mRNA were detected in 8 Burkitt's lymphoma cell lines, 10 other neoplastic cell lines, and 2 clinical samples-one derived from a patient with ALL and a second derived from a patient with relapsed myeloma. In addition, Northern analysis of fetal tissues showed upregulated expression of PBK/TOPK in fetal kidney, lung, spleen, brain, and testis. These data suggest that PBK/TOPK expression is increased in highly proliferative malignant cells and during normal fetal development.
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| Authors | M Simons-Evelyn, K Bailey-Dell, J A Toretsky, D D Ross, R Fenton, D Kalvakolanu, A P Rapoport |
| Journal | Blood cells, molecules & diseases (Blood Cells Mol Dis) 2001 Sep-Oct Vol. 27 Issue 5 Pg. 825-9 ISSN: 1079-9796 [Print] United States |
| PMID | 11783945 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | (c)2001 Elsevier Science. |
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