An immunoassay for canine pancreatic elastase 1 as an indicator for exocrine pancreatic insufficiency in dogs.

Abstract

The detection of pancreatic elastase 1 in stool samples has become the noninvasive gold standard for the diagnosis of pancreatic insufficiency in humans. Accordingly, the development of a sandwich-ELISA specific for canine pancreatic elastase 1, based on monoclonal antibodies, is presented here. The test has a detection range of 4-240 microg canine pancreatic elastase l/g feces. The intraassay coefficient of variation is 7.4%, and the interassay coefficient of variation is 7.7%. Spiking experiments show that canine elastase 1 is quantitatively detectable in fecal samples. Interestingly, the range of the elastase 1 concentration in canine feces within several days is higher as compared with humans. As the proposed cutoff of 10 microg/g is below this variation range in 96.1% of the tested samples, the effect on the test specificity is negligible. Because the test detects neither human nor bovine and porcine elastase 1, pancreatic function can be monitored without interrupting an enzyme replacement therapy.

• Authors: T Spillmann, A Wittker, S Teigelkamp, C Eim, E Burkhardt, E Eigenbrodt, A Sziegoleit
• Journal: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc (J Vet Diagn Invest) Vol. 13 Issue 6 Pg. 468-74 (Nov 2001) ISSN: 1040-6387 [Print] United States
• PMID: 11724136 (Publication Type: Evaluation Study, Journal Article)

Chemical References

• Antibodies, Monoclonal
• Pancreatic Elastase

Topics

• Animals
• Antibodies, Monoclonal
• Dog Diseases (diagnosis)
• Dogs
• Enzyme-Linked Immunosorbent Assay (veterinary)
• Exocrine Pancreatic Insufficiency (diagnosis, veterinary)
• Feces (chemistry)
• Mice
• Mice, Inbred BALB C
• Pancreatic Elastase (analysis)
• Reference Values
• Sensitivity and Specificity