Cloning of a cDNA encoding an isoform of human protein phosphatase inhibitor 2 from vascularized breast tumor.

Abstract

Using modified differential display, a gene fragment was identified as being over-expressed in human vascularized breast carcinoma when compare to its neighboring normal tissue. The differentially expressed pattern was confirmed by quantitative RT-PCR. Full-length cDNA was then cloned by both 3'-end RACE and 5'-end RACE. Analysis of the full-length cDNA of this gene reveals that this cDNA encodes an open reading frame of 615 bp, which is highly homologous to human protein phosphatase inhibitor-2, with 92% identity at the nucleotide level, and 89% identity at amino acid level. The results of this study suggest that this novel isoform of human protein phosphatase inhibitor-2 (nPPI-2) may be involved in the angiogenic switch during breast tumor development.

• Authors: I Wu, M A Moses
• Journal: DNA sequence : the journal of DNA sequencing and mapping (DNA Seq) Vol. 11 Issue 6 Pg. 515-8 ( 2001) ISSN: 1042-5179 [Print] England
• PMID: 11696978 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 3' Untranslated Regions
• 5' Untranslated Regions
• DNA, Complementary
• Neoplasm Proteins
• Protein Isoforms
• Proteins
• protein phosphatase inhibitor-2
• Phosphoprotein Phosphatases

Topics

• 3' Untranslated Regions
• 5' Untranslated Regions
• Amino Acid Sequence
• Base Sequence
• Breast Neoplasms (genetics)
• Carcinoma, Ductal, Breast (genetics)
• Cloning, Molecular
• DNA, Complementary
• Female
• Gene Expression Profiling
• Humans
• Molecular Sequence Data
• Neoplasm Proteins (genetics)
• Neovascularization, Pathologic
• Open Reading Frames
• Phosphoprotein Phosphatases (antagonists & inhibitors)
• Protein Isoforms (genetics)
• Proteins (genetics)