Concurrent naive and memory CD8(+) T cell responses to an influenza A virus.

Abstract

Memory Thy-1(+)CD8(+) T cells specific for the influenza A virus nucleoprotein (NP(366-374)) peptide were sorted after staining with the D(b)NP(366) tetramer, labeled with CFSE, and transferred into normal Thy-1.2(+) recipients. The donor D(b)NP(366)(+) T cells recovered 2 days later from the spleens of the Thy-1.2(+) hosts showed the CD62L(low)CD44(high)CD69(low) phenotype, characteristic of the population analyzed before transfer, and were present at frequencies equivalent to those detected previously in mice primed once by a single exposure to an influenza A virus. Analysis of CFSE-staining profiles established that resting tetramer(+) T cells divided slowly over the next 30 days, while the numbers in the spleen decreased about 3-fold. Intranasal infection shortly after cell transfer with a noncross-reactive influenza B virus induced some of the donor D(b)NP(366)(+) T cells to cycle, but there was no increase in the total number of transferred cells. By contrast, comparable challenge with an influenza A virus caused substantial clonal expansion, and loss of the CFSE label. Unexpectedly, the recruitment of naive Thy-1.2(+)CD8(+)D(b)NP(366)(+) host D(b)NP(366)(+) T cells following influenza A challenge was not obviously diminished by the presence of the memory Thy-1.1(+)CD8(+)D(b)NP(366)(+) donor D(b)NP(366)(+) set. Furthermore, the splenic response to an epitope (D(b)PA(224)) derived from the influenza acid polymerase (PA(224-233)) was significantly enhanced in the mice given the donor D(b)NP(366)(+) memory population. These experiments indicate that an apparent recall response may be comprised of both naive and memory CD8(+) T cells.

• Authors: S J Turner, R Cross, W Xie, P C Doherty
• Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 167 Issue 5 Pg. 2753-8 (Sep 01 2001) ISSN: 0022-1767 [Print] United States
• PMID: 11509619 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• NP protein, Influenza A virus
• Nucleocapsid Proteins
• Nucleoproteins
• Peptide Fragments
• RNA-Binding Proteins
• Viral Core Proteins

Topics

• Animals
• CD8-Positive T-Lymphocytes (immunology, transplantation)
• Female
• Immunologic Memory
• Influenza A virus (immunology)
• Influenza B virus (immunology)
• Lung (immunology, virology)
• Mice
• Mice, Congenic
• Nucleocapsid Proteins
• Nucleoproteins (immunology)
• Orthomyxoviridae Infections (immunology, virology)
• Peptide Fragments (immunology)
• Phenotype
• RNA-Binding Proteins
• Viral Core Proteins (immunology)