IgG-mediated histamine release from canine mastocytoma-derived cells.

Abstract

BACKGROUND:

Recent data suggest that normal tissue mast cells can express functional receptors for IgG under certain conditions. However, little is known about IgG receptor expression and functional consequences in mast cell neoplasms.

METHODS:

In this study, neoplastic mast cells were obtained from a dog with cutaneous mastocytoma (CM-MC) and from a dog with visceral mastocytoma (VI-MC). Both cell populations were characterized morphologically and functionally.

RESULTS:

Most cells proliferated constantly in suspension without particular supplements. Doubling times of CM-MC and VI-MC were 52.2 and 27.5 h, respectively. Both cell types were sensitive to formalin fixation, did not contain heparin and were tryptase and chymase positive. Electron microscopy showed fine granules with electron-dense content in both cell populations. The total histamine content of CM-MC and VI-MC was 0.25 and 0.10 pg/cell, respectively. Calcium ionophore A23187 and substance P induced dose-dependent histamine release, whereas compound 48/80 had no effect. Most significantly, both cell types, when sensitized with monomeric dog IgG, released histamine upon stimulation by anti-dog IgG.

CONCLUSIONS:

Dog mastocytoma-derived cells may be useful to study the regulation of neoplastic mast cell growth and differentiation, as well as IgG receptor-mediated activation in neoplastic mast cells. Further research is required to clarify the pathophysiological significance of constitutive expression of IgG receptors in neoplastic (canine) mast cells.

• Authors: T Takahashi, S Kitani, M Nagase, M Mochizuki, R Nishimura, Y Morita, N Sasaki
• Journal: International archives of allergy and immunology (Int Arch Allergy Immunol) Vol. 125 Issue 3 Pg. 228-35 (Jul 2001) ISSN: 1018-2438 [Print] Switzerland
• PMID: 11490155 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2001 S. Karger AG, Basel.

Chemical References

• Immunoglobulin G
• Ionophores
• Substance P
• Calcimycin
• Serine Endopeptidases
• Chymases
• Tryptases

Topics

• Animals
• Calcimycin (pharmacology)
• Cell Line
• Chymases
• Dog Diseases (immunology, pathology)
• Dogs (immunology)
• Female
• Histamine Release
• Immunoglobulin G (pharmacology)
• Intestinal Neoplasms (ultrastructure, veterinary)
• Ionophores (pharmacology)
• Male
• Mast Cells (drug effects, immunology, ultrastructure)
• Mast-Cell Sarcoma (ultrastructure, veterinary)
• Microscopy, Electron
• Serine Endopeptidases (analysis)
• Skin Neoplasms (ultrastructure, veterinary)
• Substance P (pharmacology)
• Tryptases
• Tumor Cells, Cultured