Abstract |
Inoculation with anti-Thy-1 antibodies (Abs) in rats induces glomerulonephritis resembling human mesangiolytic and/or mesangioproliferative diseases. Some anti-Thy-1 monoclonal Abs (mAbs) react with both mesangial and glomerular endothelial cells, whereas others react solely with mesangial cells in rat kidney. These findings suggest that the rat Thy-1 molecule possesses at least 2 variant forms, including a mesangial and a vascular endothelial isoform. However, anti-Thy-1 mAbs with specific reactivity with glomerular endothelial cells have not been available. We describe here a unique anti-rat Thy-1 mAb, TM78-8. The epitope for TM78-8 is closely related, but not identical, to that for OX-7, a commercially available anti-rat Thy-1 mAb. Immunoblotting, immunohistochemistry and immunoelectron microscopy confirm that TM78-8 reacts exclusively with Thy-1 antigens on the surface of vascular endothelial cells in rat glomeruli. TM78-8 may be a suitable marker for rat glomerular endothelial cells as well as for the vascular endothelial isoform of the rat Thy-1 molecule. Intravenous injection of TM78-8 did not induce glomerulonephritis in rats, whereas OX-7 did, indicating that TM78-8 is not nephritogenic. This finding also corresponds with the current consensus that Thy-1 antigens expressed on mesangial cells play an essential role in the development of Thy-1 nephritis.
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Authors | A Ishizu, Y Ogawa, H Ishikura, T Yoshiki |
Journal | Acta histochemica
(Acta Histochem)
Vol. 103
Issue 3
Pg. 279-86
(Jul 2001)
ISSN: 0065-1281 [Print] Germany |
PMID | 11482374
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- Thy-1 Antigens
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Topics |
- Animals
- Antibodies, Monoclonal
(immunology)
- Endothelium, Vascular
(cytology, immunology)
- Flow Cytometry
- Glomerulonephritis
(immunology)
- Humans
- Kidney Glomerulus
(blood supply)
- Proteinuria
(chemically induced, immunology)
- Rats
- Rats, Wistar
- Thy-1 Antigens
(immunology, metabolism)
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