Abstract |
Somatic antigen of Ascaris lumbricoides was purified to homogeneity (molecular mass, 34 kDa) by ammonium sulfate fractionation and successive chromatographic procedures, namely, gel permeation, ion exchange, and high-performance gel permeation liquid chromatographies. The antigen showed strong binding with immunoglobulin G ( IgG) in Ascaris-infested patients and was cross-reactive with IgE and IgG in patients infested with other nematodes. It reacted specifically with IgG4 (P < 0.001) in 63 Ascaris-infested patients, which represented 65% of the total IgG response, though cross-reactivity with IgG1, IgG2, and IgG3 subclasses was observed, indicating the unique specificity of this test system and its potential utility in the serodiagnosis of ascariasis.
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Authors | T Bhattacharyya, A Santra, D N Majumder, B P Chatterjee |
Journal | Journal of clinical microbiology
(J Clin Microbiol)
Vol. 39
Issue 8
Pg. 2991-4
(Aug 2001)
ISSN: 0095-1137 [Print] United States |
PMID | 11474031
(Publication Type: Evaluation Study, Journal Article)
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Chemical References |
- Antibodies, Helminth
- Antigens, Helminth
- Immunoglobulin G
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Topics |
- Adolescent
- Adult
- Animals
- Antibodies, Helminth
(blood)
- Antigens, Helminth
(immunology, isolation & purification)
- Ascariasis
(diagnosis, parasitology)
- Ascaris lumbricoides
(immunology)
- Child
- Enzyme-Linked Immunosorbent Assay
(methods)
- Female
- Humans
- Immunoglobulin G
(blood)
- Male
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