Effect of retinoic acid on the Ca2+-independent phospholipase A2 in nuclei of LA-N-1 neuroblastoma cells.

Abstract

LA-N-1 neuroblastoma cell cultures contain Ca2+-independent phospholipases A2 hydrolyzing phosphatidylethanolamine and ethanolamine plasmalogens. These enzymes differ from each other in their molecular mass, substrate specificity, and kinetic properties. Subcellular distribution studies have indicated that the activity of these phospholipases is not only localized in the cytosol but also in non-nuclear membranes and in nuclei. The treatment of LA-N-1 neuroblastoma cell cultures with retinoic acid results in a marked stimulation of Ca2+-independent phospholipases A2 hydrolyzing phosphatidylethanolamine and plasmenylethanolamine. The increase of the activities of both enzymes was first observed in nuclei followed by those present in the cytosol. No effect of retinoic acid on either phospholipase activity could be observed in non-nuclear membranes. The stimulation of these enzymes may be involved in the generation and regulation of arachidonic acid and its metabolites during differentiation.

• Authors: P Antony, L Freysz, L A Horrocks, A A Farooqui
• Journal: Neurochemical research (Neurochem Res) Vol. 26 Issue 1 Pg. 83-8 (Jan 2001) ISSN: 0364-3190 [Print] United States
• PMID: 11358286 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Phosphatidylethanolamines
• Plasmalogens
• phosphatidal ethanolamines
• Tretinoin
• Phospholipases A
• Phospholipases A2
• Calcium

Topics

• Calcium (physiology)
• Cell Nucleus (drug effects, enzymology)
• Cytosol (enzymology)
• Humans
• Hydrolysis (drug effects)
• Neuroblastoma (enzymology)
• Phosphatidylethanolamines (metabolism)
• Phospholipases A (metabolism)
• Phospholipases A2
• Plasmalogens (metabolism)
• Tretinoin (pharmacology)
• Tumor Cells, Cultured