In vitro transformation of rat renal cells by treatment with ferric nitrilotriacetate.

Abstract

Administration of ferric nitrilotriacetate (Fe-NTA) in vivo causes acute renal tubular injury and finally induces renal cell carcinoma. There is accumulating evidence that these processes involve free radicals generated by Fe-NTA. To study the mechanism of renal carcinogenesis by Fe-NTA, we attempted to induce malignant transformation of primary cultured renal cells by treatment with Fe-NTA. When primary cultured renal cells (PRC) were treated continuously with Fe-NTA, all of the PRC died without transformation. On the other hand, when PRC were treated intermittently with Fe-NTA, transformed epithelial colonies were observed at 3 weeks after the first treatment. The established transformed cell line (RK523) showed drastic morphological transformation, grew in soft agar, and formed tumors when transplanted into athymic nude mice. These results indicate that the balance between cytotoxicity and mutagenecity is important for Fe-NTA induced transformation. The RK523 cell line may be a useful model for studying renal carcinogenesis in vitro.

• Authors: C Kakehashi, M Mori, T Kawabata, S Okada
• Journal: Acta medica Okayama (Acta Med Okayama) Vol. 55 Issue 2 Pg. 97-103 (Apr 2001) ISSN: 0386-300X [Print] Japan
• PMID: 11332205 (Publication Type: Journal Article)

Chemical References

• Carcinogens
• Ferric Compounds
• Nitrilotriacetic Acid
• ferric nitrilotriacetate

Topics

• Animals
• Carcinogens (pharmacology)
• Cell Survival
• Cell Transformation, Neoplastic (chemically induced)
• Ferric Compounds (administration & dosage, pharmacology)
• Humans
• Kidney (cytology, drug effects, ultrastructure)
• Kidney Neoplasms (chemically induced, ultrastructure)
• Mice
• Mice, Nude
• Neoplasm Transplantation
• Nitrilotriacetic Acid (administration & dosage, analogs & derivatives, pharmacology)
• Rats
• Tumor Cells, Cultured