Abstract |
Ovine pulmonary carcinoma (OPC) is a contagious neoplasm of alveolar epithelial type II (ATII) or Clara cells caused by a type D/B chimeric retrovirus, jaagsiekte sheep retrovirus (JSRV). Here we report the isolation, sequencing, pathogenicity, and integration site of a JSRV provirus isolated from a sheep lung tumor cell line (JS7). The sequence of the virus was 93 to 99% identical to other JSRV isolates and contained all of the expected open reading frames. To produce virions and test its infectivity, the JS7 provirus (JSRV(JS7)) was cloned into a plasmid containing a cytomegalovirus promoter and transfected into 293T cells. After intratracheal inoculation with virions from concentrated supernatant fluid, JSRV-associated OPC lesions were found in one of four lambs, confirming that JSRV(JS7) is pathogenic. In JS7-cell DNA, the viral genome was inserted in the protein-coding region for the surfactant protein A (SP-A) gene, which is highly expressed in ATII cells, in an orientation opposite to the direction of transcription of the SP-A gene. No significant transcription was detected from either the viral or the SP-A gene promoter in the JS7 cell line at passage level 170. The oncogenic significance of the JSRV proviral insertion involving the SP-A locus in the JS7 tumor cell line is unknown.
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Authors | J C DeMartini, J V Bishop, T E Allen, F A Jassim, J M Sharp, M de las Heras, D R Voelker, J O Carlson |
Journal | Journal of virology
(J Virol)
Vol. 75
Issue 9
Pg. 4239-46
(May 2001)
ISSN: 0022-538X [Print] United States |
PMID | 11287573
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- DNA, Viral
- Proteolipids
- Pulmonary Surfactant-Associated Protein A
- Pulmonary Surfactant-Associated Proteins
- Pulmonary Surfactants
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Topics |
- Animals
- Base Sequence
- Binding Sites
- Cell Line, Transformed
- DNA, Viral
- Humans
- Jaagsiekte sheep retrovirus
(genetics, isolation & purification, pathogenicity)
- Lung Neoplasms
- Molecular Sequence Data
- Proteolipids
(genetics)
- Proviruses
(genetics, isolation & purification)
- Pulmonary Adenomatosis, Ovine
(virology)
- Pulmonary Surfactant-Associated Protein A
- Pulmonary Surfactant-Associated Proteins
- Pulmonary Surfactants
(genetics)
- Sheep
- Tumor Cells, Cultured
- Virus Integration
(genetics)
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