Abstract | BACKGROUND: METHODS: Employing 22 pair-matched benign and malignant tissue samples that were obtained from the same patients with prostate carcinoma, the expression of 5-LO was determined using reverse transcriptase-polymerase chain reaction, immunoblotting, and immunohistochemistry and by measuring the levels of 5-hydroxyeicosatetraenoic acid (5-HETE) by radioimmunoassay. RESULTS: The mean level of 5-LO mRNA was six-fold greater (P < 0.001) in malignant tissue compared with benign tissue. The immunoblot analysis demonstrated that, compared with benign tissue, 5-LO protein was overexpressed in 16 of 22 samples examined and was 2.6 fold greater (P < 0.001) in malignant tissue. Immunohistochemical studies further verified 5-LO up-regulation in malignant tissue that was not present in benign tissue. The levels of 5-HETE, which is a metabolic product of arachidonic acid, was found to be 2.2-fold greater (P < 0.001) in malignant tumor tissue compared with benign tissue. CONCLUSIONS: To the authors' knowledge, this is the first in vivo study showing overexpression of 5-LO in patients with prostate carcinoma. This study suggests that inhibitors of arachidonic acid pathway in general and selective 5-LO inhibitors in particular may be useful for prevention or therapy in patients with prostate carcinoma.
|
Authors | S Gupta, M Srivastava, N Ahmad, K Sakamoto, D G Bostwick, H Mukhtar |
Journal | Cancer
(Cancer)
Vol. 91
Issue 4
Pg. 737-43
(Feb 15 2001)
ISSN: 0008-543X [Print] United States |
PMID | 11241241
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
|
Copyright | Copyright 2001 American Cancer Society. |
Chemical References |
- Hydroxyeicosatetraenoic Acids
- RNA, Messenger
- 5-hydroxy-6,8,11,14-eicosatetraenoic acid
- Arachidonate 5-Lipoxygenase
|
Topics |
- Adenocarcinoma
(enzymology, metabolism)
- Aged
- Arachidonate 5-Lipoxygenase
(metabolism)
- Humans
- Hydroxyeicosatetraenoic Acids
(metabolism)
- Immunohistochemistry
- Male
- Middle Aged
- Prostatic Neoplasms
(enzymology, metabolism)
- RNA, Messenger
(analysis)
- Radioimmunoassay
- Reverse Transcriptase Polymerase Chain Reaction
|