Serum S-100beta protein predicts brain injury after hypothermic circulatory arrest in pigs.

Abstract

OBJECTIVE:

Serum S-100beta protein is suggested to be a neurobiochemical marker of brain injury after cardiac and aortic arch surgery. The aim of the present study was to investigate the predictive value of S-100beta protein with respect to histopathological analysis of the brain after a prolonged period of hypothermic circulatory arrest (HCA).

METHODS:

Eighteen pigs (21 to 31 kg) underwent a 75 min period of HCA at 20 degrees C. Serum concentrations of S-100beta were assayed in mixed venous blood before and 2, 4, 7 and 20 h after HCA. A semiquantitative post-mortem histopathological analysis scoring all main regions of the brain was carried out in every animal.

RESULTS:

All animals were stable during and after cardiopulmonary bypass (CPB) and survived at least to the first postoperative day. Ten of the 18 animals survived 7 days after surgery and were electively sacrificed. Animals with severe histopathological injury showed higher serum S-100beta protein levels at every time point after HCA. The strongest correlation between the total histopathologic score and serum S-100beta levels was found at 7 h after HCA (tau = 0.422 and p = 0.023).

CONCLUSION:

Serum S-100beta protein levels correlate with histopathological injury after a prolonged period of HCA in pigs. This finding supports the results of previous studies suggesting the potential accuracy of S-100beta in the prediction of brain injury after cardiac surgery.

• Authors: M Pokela, V Anttila, J Rimpiläinen, J Hirvonen, V Vainionpää, K Kiviluoma, P Romsi, A Mennander, T Juvonen
• Journal: Scandinavian cardiovascular journal : SCJ (Scand Cardiovasc J) Vol. 34 Issue 6 Pg. 570-4 (Dec 2000) ISSN: 1401-7431 [Print] England
• PMID: 11214009 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Calcium-Binding Proteins
• Nerve Growth Factors
• S100 Calcium Binding Protein beta Subunit
• S100 Proteins

Topics

• Animals
• Brain (pathology)
• Brain Damage, Chronic (blood, pathology)
• Calcium-Binding Proteins (blood)
• Cardiopulmonary Bypass (adverse effects)
• Female
• Heart Arrest, Induced
• Hypothermia, Induced
• Nerve Growth Factors (blood)
• Predictive Value of Tests
• S100 Calcium Binding Protein beta Subunit
• S100 Proteins
• Swine